实验大鼠和小鼠多种病毒的血清学检测结果分析

Testing of Multiple Viral Antibodies in Laboratory Rats and Mice

目的评估近期大鼠和小鼠的病毒感染情况,为我国大、小鼠质量控制和标准化提供参考。方法应用酶联免疫吸附试验(ELISA)对2012年至2013年国内36家单位送检的小鼠和大鼠血清进行病毒抗体检测,检测出现阳性的样品再用免疫荧光试验(IFA)进行复检。结果检测小鼠病毒抗体18项,出现阳性结果的有9项,小鼠诺如病毒和小鼠肝炎病毒阳性率最高,分别是42.2%和19.9%。检测大鼠病毒抗体14项,出现阳性结果的有8项,大鼠细小病毒有5项,阳性率最高,达13.6%—34.4%。结论我国大、小鼠病毒监测、净化和控制工作仍需加强。

Objective To evaluate serologic prevalence of viral antibody in laboratory rats and mice and provide reference for the animal health monitoring and standardization of laboratory rodents. Method Samples from rats and mice submitted in 2012 and 2013 from 36 institutions were tested by enzyme-linked immune sorbent assay （ELISA）, and positive samples were confirmed by immune fluorescence assay （IFA）. Result Nine of 19 antibodies were positive from mouse samples and the most prevalent viruses are mouse hepatitis virus and mouse norovirus, with a prevalence of 20.44% and 19.28% , respectively. Eight of 14 antibodies were positive from rat samples and 5 items of the parvoviridae appeared to be the most prevalent viruses, with a positive ratio from 19.39% to 42. 62%. Conclusion The virus monitoring and quality control for mice and rats should be strengthened.