6—8月龄健康Beagle犬血液微量元素测定

Detection on Blood Trace Elements of 6- 8 Months Beagle Dogs

目的建立本繁育饲养基地正常Beagle犬血液中主要微量元素指标正常参考值范围,为医学研究提供参考基础数据。方法选用普通级健康Beagle犬60只(雄性30只,雌性30只),6—8月龄,体质量6.5—8.5 kg,前肢内侧隐静脉采血,使用TC-3010A型微量元素分析仪及配套试剂,分别测定血液中锌、铁、锰、钙、镁、铅、铜、镉等8种微量元素,将数据进行统计分析。结果分别得出了雌雄Beagle犬血液中锌、铁、钙、镁、铜、铅六种微量元素指标参考值的均值及标准差,未测定出锰和镉。结论血液中铁、钙、铅指标雌雄间比较差异不显著(P>0.05),锌、镁和铜指标雌雄间比较差异显著(P<0.05)。

Objective Normal reference data of blood main trace element of 6 - 8 months Beagle Dogs in Sichuan Institute of Musk Deer Breeding were detected. It will provide basic data for medical research. Method Blood of 60 healthy Beagle dogs （30 males and 30 females） at the age of 6 -8 months were collected by forelimb medial saphenous vein. Blood trace elements including zinc [ Zn 1, iron [Fe], manganese [ Mn], magnesium [ Mg], lead [Pb], copper [Cu], cadmium[Cd] and calcium[Ca] were evaluated by TC -3010A -type trace element analyzer. The data were analyzed using statistical analysis. Result Mean and standard deviation of normal reference data in Beagle dogs＇blood trace elements including zinc [ Zn], iron [ Fe], magnesium [ Mg], lead [Pb], copper [Cu] and calcium[Ca] were obtained. Mn and Cd were not detected in blood. Conclusion There are no significant difference of Fe, Ca and Pb in blood between males and females （P 〉 0.05）. But the index of Zn, Mg and Cu have significant difference （P 〈 0.05）. These knowledge was the first time to study blood trace element of 6 -8 months Beagle dogs, and its results have a good referential value.