马尾松土贡种源松针挥发油化学成分GC/MS分析

GC /MS Analysis of Chemical Composition of Pine Needle Oil from Pinus massoniana Tugong Provenance

用挥发油提取器提取马尾松土贡种源松针中挥发油,利用气相色谱-质谱(GC-MS)分析其中的化学成分,采用GC峰面积归一化法定量,并与马尾松普通种源松针油做对比。结果表明,土贡种源和普通种源挥发油得油率分别为0.52%、0.60%,各分出69、75个成分,鉴定其中23个,分别占挥发油总量的92.65%、94.56%,其挥发油主要成分为α-蒎烯(37.673%、46.715%)、莰烯(3.724%、3.015%)、β-蒎烯(14.969%、12.978%)、石竹烯(8.242%、10.212%),除α-蒎烯含量相差9.042%以外,其他成分含量相差不大。

The essential oil of Tugong Provenance and ordinary type Pinus massoniana was extracted by the volatile oil extractor , and the chemical composition of essential oil was analyzed and compared by gas chromatography -mass spectrometry （ GC-MS） by using the peak area normalization method .The results showed that the yield of essential oil of these two provenances were 0.52 % and 0.60 % respectively .23 constituents were identified from 69 and 75 chromatographic peaks respectively , and the relative content up to 92.65%, 94.56% respectively .The major constituents were α-pinene （ 37.673 %, 46.715 %） , camphene （ 3.724 %, 3.015 %） , β-pinene （14.969 %, 12.978 %）, caryophyllene （8.242 %, 10.212 %）.The major constituents were similar except theα-pinene content difference with 9.042 %.