摘要
目的检测Snail、E-cadherin及Vimentin在上皮性卵巢癌组织中的表达,探讨Snail介导的上皮间质转化(EMT)在卵巢癌发生、发展及转移中的作用。方法采用免疫组化法分别检测Snail、E-cadherin、Vimentin在48例卵巢浆液性腺癌、卵巢交界性浆液性肿瘤及卵巢浆液性腺瘤中的表达,探讨EMT相关因子表达强度的相关性及与临床病理特征之间的关系。结果 (1)Snail、Vimentin在卵巢浆液性腺癌中的表达率为(68.75%/66.67%),高于卵巢交界性浆液性腺瘤的(41.67%/37.50%)及卵巢浆液性腺瘤的(25.00%/18.75%),结果均具有显著的统计学差异(P<0.05),E-cadherin在卵巢浆液性腺癌中的表达率为27.08%,低于与卵巢交界性浆液性腺瘤的54.17%及卵巢浆液性腺瘤的75.00%,结果均具有统计学差异(P<0.05)。而卵巢交界性浆液性腺瘤组与卵巢浆液性腺瘤组比较,三种蛋白的表达均无统计学差异(P>0.05)。(2)Snail、E-cadherin及Vimentin的表达高低与FIGO分期、分化级别、有无淋巴结转移及腹膜种植有关。(3)在卵巢浆液性腺癌中,Snail与Vimentin的表达呈正相关(r=0.477,P<0.05),Snail与E-cadherin的表达呈负相关(r=-0.601,P<0.05),而E-cadherin与Vimentin表达的相关性不明显(r=-0.206,P>0.05)。结论在上皮性卵巢癌组织中,Snail、Vimentin表达上调,E-cadherin表达下调,提示Snail介导的EMT可能与卵巢癌的发生、发展及浸润转移有关。
Objective To Study the effect on Snail mediated epithelial mesenchymal transition （EMT） in ovarian cancer occurrence, development and metastasis. Methods Immunohistochemical staining was detected Snail, E-cadherin,in 48 cases of ovarian serous adenocarcinoma,ovarian serous borderline tumors and ovarian serous adenomas Vimentin,investigate the correlation between the expression of EMT -related factors and the strength and relationship between clinicopathological features. Results（1）The expression of Snail,Vimentin in ovarian serous adenocarcinoma was 68.75% and 66.67%,higher than in borderline serous ovarian tumors （41.67% and 37.50） and in ovarian serous adenomas（ 25.00%and 18.75%）,with significant difference（P〈0.05）,the expression of E-cadherin in ovarian serous adenocarcinoma of the rate of 27.08%,lower than in borderline serous ovarian tumors（54.17%） and in ovarian serous adenomas（75.00%）,with significant difference（P 〈 0.05）. Between the borderline ovarian serous cystadenoma and ovarian serous adenoma group,there was statistical difference in the expression of the three proteins（P ＆gt; 0.05）.（2）The expression level of Snail and FIGO, E-cadherin and Vimentin staging,differentiation level,there was no lymph node metastasis and peritoneal implants.（3）in ovarian serous adenocarcinoma, the expression of Snail positively correlated with Vimentin （r=0.477,P 〈 0.05）,the expression of Snail was negatively correlated with E-cadherin（r=-0.601,P 〈 0.05）,and the expression of E-cadherin and Vimentin correlation is not obvious（r=-0.206,P〈 0.05）. Conclusion In epithelial ovarian cancer,Snail,Vimentin upregulation,E-cadherin downregulation,suggesting that Snail-mediated EMT may be transferred with ovarian cancer,development and infiltration.
出处
《中国医药科学》
2014年第7期9-13,20,共6页
China Medicine And Pharmacy
