新型掺锶硫酸钙材料的成骨细胞生长活性研究

Growth activity of osteoblast on a novel strontium incorporated calcium sulfate

目的:评价新型掺锶硫酸钙材料的成骨细胞生长活性,并与硫酸钙材料进行比较。方法:通过成骨细胞在掺锶硫酸钙和硫酸钙材料表面的直接接种,于第1,3,5天观察成骨细胞的增殖活性,分别在5 d后检测细胞碱性磷酸酶活性和骨钙素活性,并用扫描电子显微镜观察细胞生长微观形态结构。结果:成骨细胞在掺锶硫酸钙材料表面旺盛生长。掺锶硫酸钙材料表面的细胞数量显著高于普通硫酸钙组(P<0.05)。其碱性磷酸酶活性较对照组高出57.8%,两者差异有统计学意义(P<0.05)。成骨细胞的骨钙素水平较后者高出40.2%(P<0.05)。掺锶硫酸钙材料表面成骨细胞呈多角形或梭形生长,胞浆丰富,状态活跃。结论:掺锶硫酸钙材料具有优良的成骨细胞生长活性,有望成为一种理想的骨替代材料。

Objective ：To investigate the growth activity of osteoblast on a novel strontium incorporated calcium sulfate and make comparison with normal calcium sulfate material. Methods： Osteoblast was inoculated on samples and cell prolifera- tion was measured on the 1st, 3rd, 5th days, and the activities of ALP and osteocalcin were observed on the 5th day. And mi- crocosmic morphology of osteoblast was observed by scanning electron microscopy （SEM）. Results：Osteoblast grows robustly on tested material. Cell quantity on the surface of novel material was obviously higher than normal calcium sulfate material （P〈 0.05）. The activity of ALP and osteocalcin on novel material was 57.8% and 40.2% higher than on normal calcium sulfate ma- terial respectively（P〈0.05）. On strontium incorporated surface, osteoblast spread well. Cells were polygonal with abundant cy- toplasm and the morphology was active. Conclusion：Strontium incorporated calcium sulfate can sustain robust growth activity of osteoblast, which is promising to be used for bone substitute materials.