光动力治疗对尖锐湿疣HPV DNA载量的影响

Effect of photodynamic therapy on HPV DNA load in condyloma acuminatum tissue

目的：确定光动力治疗对尖锐湿疣（ CA）人乳头瘤病毒（ HPV）的影响。方法：采用实时荧光定量聚合酶链反应（ FQ-PCR）检测65例CA患者光动力治疗前、治疗3次后HPV DNA拷贝数。随访3个月观察复发情况，并分析复发与HPV DNA载量的关系。结果：3次治疗后HPV DNA拷贝数（3.10×10^3）较治疗前（5.12×10^6）显著下降（P〈0.05）；复发患者（12例）治疗前平均拷贝数（4.62×10^7）高于未复发患者（6.75×10^4）（P〈0.05）。结论：光动力治疗CA能显著降低HPV病毒载量；患者疣体中HPV DNA载量与复发有关。

Objective：To determine the effect of photodynamic therapy on human papilloma virus （ HPV） DNA load in the lesion of condyloma acuminatum （ CA） . Methods：The number of HPV DNA copy in 65 pa-tients with （ CA） were detected by Real-time fluorescence quantitative polymerase chain reaction （ FQ-PCR） before the treatment and after 3 times treatment. The patients were followed-up for relapse after 3 months treat-ment, The correlation between the relapse and the number of HPV DNA copy were analyzed. Results：The av-erage HPV DNA copy number was （ 5. 12 × 10^6 ） before the treatment and the HPV DNA copy number was （3.10×10^3） after the treatment, with a significant difference （P〈0.05）. A total of 12 patients were relapsed. The average HPV DNA copy number in the relapsed patients was （4.62×10^7） before the treatment, which was higher than （6.75×10^4） in the non-relapsed patients before the treatment （P〈0.05）. Conclusion：Photody-namic therapy can significantly reduce HPV load in the lesion of CA. The recurrence of CA was correlated with the HPV-DNA load before the treatment.