慢性阿片类耐受、戒断状态下下丘脑-垂体-性腺轴改变及温阳益气活血复方作用的临床与实验研究

The Changes in Hypothalamus - pituitary - testicle Axis Chronic Mor Dependence - abstinence Rats and Patients and Clinical and Experimental Study of "Warming Yang, Replenishing Energy, Invigorating Blood Circulation and Eliminating Blood Stasis Co

目的:研究机体下丘脑-垂体-睾丸轴在阿片类耐受、戒断过程中的变化,探讨温阳益气活血复方抗戒断症状的作用机理;方法:建立慢性Mor依赖大鼠模型,设立正常组、自然戒断组和复方治疗组,对各组大鼠的睾丸、附睾组织进行光镜观察;运用放免法检测成瘾患者和大鼠血清FSH、LH、T、PRL等的变化;结果:发现耐受和戒断组大鼠的睾丸、附睾出现退行性改变,血清LH、T明显降低,PRL显著升高;成瘾患者血清性激素水平明显紊乱,温阳益气活血复方能逆转成瘾大鼠睾丸、附睾退行性改变,恢复机体紊乱的性激素水平;结论:阿片类依赖确能引起机体睾丸轴的机能和实质性改变,出现“肾阳虚损”未老先衰的退行性变化,且此变化在戒断后的一段时间内仍不能复常,温阳益气活血复方可逆转这种改变。

To explore the mechanisms of 'Wanning Yang, replenishing energy, invigorating blood circulation and eliminating blood stasis compound prescription treating opiate dependence - abstinence syndromes, the changes of hypothalamus - pituitary - testicle axis were observed in chronic Mor - dependence rats when they were being formed and being abstined. Chronic Mor - dependence rats models were established and pathological injuries accrued in testicle and epididymis in the rat models were obverled by light microscope, the compared with that in normal group, abstinence group and CCD treatment group. FSH, LH, T and PRL in the serum of addicted patient and rats were detected by radioimmunology. Dependence - abstinence rats had degeneration changes in testicle and epididymis, apparently decreased T and LH in serum while PRL lifted obviously; Addicted patients had obviously sex hormone disorders in serum; The compound Chinese drug can adjust chaos of sex hormone and reverse the degeneration changes in testicle and epididymis. Opiate - dependence can cause function and histopathology changes in testicle axis of dependent body and prematurely senile degeneration changes in 'Yang deficiency', which could not recover for a long time, but Warm Yang, replenish energy, invigorate blood circulation and eliminate blood stasis compound prescription can reverse them.