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体内直接克隆大片段DNA的研究进展 被引量：1

Progress of in vivo Direct Cloning of Large DNA Fragments
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摘要 基因组序列的功能分析以及代谢途径的构建改造等都需要克隆目的 DNA。获得大片段DNA序列的方法有构建和筛选基因文库,PCR扩增,体外大片段DNA合成和组装等,但体内重组直接克隆的方法在操作、克隆长片段和应用等方面更具优势。介绍了Red/ET重组介导的大片段DNA体内直接克隆的主要方法及其应用。 Cloning of interest DNA is necessary for functional analysis of genome sequences and research on metabolically engineered pathways of modern producer strains. Methods to precisely clone large DNA fragments include library constructing and screening, PCR amplification and various different DNA assembly methods in vitro. In addition, direct cloning by homologous recombination in vivo has more advantages in the cloning and engineering of long DNA sequences. The main methods of direct cloning of large DNA fragments in vivo via Red/ ET recombination system and its applications were introduced.
作者 朱莹 倪孟祥 方宏清
机构地区 中国药科大学生命科学与技术学院 军事医学科学院生物工程研究所
出处 《中国生物工程杂志》 CAS CSCD 北大核心 2014年第4期95-100,共6页 China Biotechnology
基金 国家重点基础研究发展计划(2011CBA00800) 国家自然科学基金(30870049 81373286)资助项目
关键词 直接克隆 RED ET 体内重组 Direct cloning Red/ET In vivo recombination
分类号 Q819 [生物学—生物工程]
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参考文献30

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中国生物工程杂志
2014年 第4期

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