Fmr1基因在大鼠快速眼动睡眠剥夺后脑皮质、海马和丘脑区的表达

Expressions of gene Fmr1 in rat cortex,hippocampus and thalamus areas after the rapid eyes movement sleep deprivation

目的探讨快速眼动(REM)睡眠剥夺过程中Fmr1基因在大鼠皮质、海马和丘脑区的表达及变化。方法采用改良多平台水环境法(MMPM)制作大鼠睡眠剥夺模型,采用免疫组织化学法及RT-PCR方法检测Fmr1基因的表达变化。结果在皮质和丘脑中,与CC组和TC组相比,SD1d和SD2d组的Fmr1基因表达无明显变化,SD3d组开始增高(P<0.05),SD5d、SD7d组和SD9d组显著增高(P<0.01);在海马中,与CC组和TC组相比,SD1d和SD2d组的Fmr1基因表达无明显变化,SD3d组开始降低(P<0.05),SD5d、SD7d组和SD9d组显著降低(P<0.01)。结论 Fmr1基因在大鼠睡眠剥夺第3天开始表达发生变化,在皮质和丘脑中表达增高,在海马中表达降低。

Objective To investigate the expression of gene Fmr1 in rats cortex, hippocampus and hypothalamus areas after the rapid eyes movement （ REM ） sleep deprivation .Methods Using the modified multiple platform method （MMPM）, 126 rats were randomly and averagely divided into three groups , the normal control group （ CC）, the environmental control group （TC） and the sleep deprivation group （SD）.Each group was detected on day 1, day 2, day 3, day 5, day 7, and day 9, and the sample tissues were extracted from 7 rats at each time point.Immunohistochemistry and RT-PCR were operated to analysis the expression of gene Fmr 1.Results The expressions of gene Fmr1 were increased gradually in the cortex and thalamus of the SD group after 3 days （ P 〈0.05 ） , and the expressions in the CC and TC groups had no significant difference （P 〉0.05）.The expressions of gene Fmr1 were decreased gradually in hippocampus for SD after 3 days （ P 〈0.05 ） , and that in the CC and TC groups had no significant difference （ P 〉0.05 ） . Conclusion The expressions of gene Fmr 1 were increased gradually in the cortex and thalamus but decreased in the hippocampus in the SD group after 3 days.