部分献血者和受血者Rh阴性血清学表型调查

Investigation of Rh negative phenotype in blood donors and recipients of Zhoukou district

目的了解河南省周口地区Rh阴性血各血清学表型的分布。方法对无偿献血者及临床受血者筛查Rh阴性标本用间接抗人球蛋白实验进行阴性确认,并用抗D、抗C、抗c、抗E和抗e试剂进行血清学表型检测。结果 103 756例标本中筛查出Rh阴性482例(0.46%),经间接抗人球蛋白法确认阴性452例(0.44%),其表型分布以ccdee型273例(60.40%)为最常见,其次为Ccdee型95例(21.02%),CcdEe型36例(7.96%),ccdEe型24例(5.31%)和CCdee型24例(5.31%)。结论该项调查初步了解了河南省周口地区Rh血型的血清学表型分布,建立了RhD阴性血型血清学表型数据库,对于临床输血安全和防治新生儿溶血病具有重要意义。

Objective To understand the distribution of local Rh negative blood phenotypes in Zhoukou district of Henan, China Methods A ttal of 103756 blood samples from blood donors and recipients was screened for the Rh negative serological phenotype, which was confirmed by indirect antiglobulin test and tested with anti D, anti C, anti C, anti E and anti e reagent phenotypes. Results Of 103 756 cases,482（0.46%） Rh negative patients were screened out and 452（0.44%） cases were confirmed by indirect antiglobulin method with Rh negative serological phenotype of ccdee in 273 （60.40%） cases,Ccdee in 95（21.02%） cases,CedEe in 36（7. 96%） eases,ccdEe in 24（5.31%） cases and CCdee in 24 cases（5.31%）. Conclusion The investigation shows that the RhD negative blood group phenotype database describing the serological phenotype distribution of Rh blood group in Zboukou area has been established preliminarily, which is of great significance for clinical blood transfusion safety and prevention of hemolytic disease of the newborn.