无滑膜肌腱滑膜化的组织工程学研究

The Tissue Engineering Research on Synovialization of Non synovial Membrane Tendon

我们将兔趾腱鞘内滑膜组织进行体外培养 ,经原代培养和传代培养 ,制成滑膜细胞悬液 ,再把兔腱鞘外无滑膜肌腱段放入滑膜细胞悬液中混合培养。经光镜、扫描电镜和免疫组织化学检测 ,结果显示 ,滑膜细胞爬行并覆盖了无滑膜肌腱段的表面 。

The synovial membrane tissue within the flexor digital tendon sheath of the rabbit's toes was cultured in vitro. The synovial cells suspension of the subculture was cultured with the segments of the non-synovial membrane tendon of the rabbit's toes. Under the light microscopy, scanning electronic microscopy and immunohistochemical examination, the result showed that the synovial cells crawled and covered the surface of the segments of the non-synovial membrane tendons. It is suggested that the non-synovial membrane tendon could transform into a synovial membrane tendon.