组织因子途径抑制因子对生物材料表面血小板粘附的影响

Effect of Tissue Factor Pathway Inhibitor on the Adhesion of Platelet onto Polyethylene and Polyvinylchloride Membranes

组织因子途径抑制因子 (tissue factor pathway inhibitor,TFPI)是组织因子凝血途径的主要抑制因子 ,具有抑制组织因子、凝血因子 VIIa、和 Xa的功能。我们以前的研究显示 TFPI在体外可以明显延长被修饰材料表面的凝血时间 ,在体内显著减少材料表面的血栓形成。本文观察了 TFPI包被对聚乙烯和聚氯乙烯材料表面血小板粘附的影响。结果显示 ,通过 TFPI处理后 ,上述两种材料表面的血小板粘附数目较对照组明显减少 ,提示

Tissue factor pathway inhibitor (TFPI) is the major inhibitor of the extrinsic pathway of the blood coagulation cascade. Our previous study showed that TFPI could obviously prolong the blood coagulation time on the surface of polyethelene (PE) and polyvinylchloride (PVC) membranes in vitro and decrease the generation of thrombus on Dacron membrane in vivo. In the present study, the effect of TFPI on the adhesion of platelet onto PE and PVC membranes was investigated. PE or PVC membrane was cut into small pieces and incubated in 96-well culture plate with GST or GST-TFPI fusion protein at 4°C over night. Freshly collected rabbit blood was mixed with 3.8% sodium citrate. Platelet-rich plasma was separated by centrifugation and then incubated with the treated membranes at 37°C for 30 minutes. The membranes were then washed, fixed, and freeze-dehydrated Adhesion of platelet was observed through scanning electronic microscope. The result showed that GST-TFPI treatment significantly decreased the number of platelet adhered on the membrane when compared with the GST and control group, and it suggested that TFPI might have a great potential to be used as an anticoagulant for improving the hemocompatibility of biomaterials.