醒脑静对颅脑创伤的保护作用

Protective effect of Xingnaojing injection on traumatic brain injury

目的:探讨醒脑静对颅脑损伤大鼠的保护作用及其机制。方法:健康雄性成年SD大鼠63只,随机分为3组(n=21):假手术组、模型组、醒脑静组。模型组与醒脑静组均采用自由落体撞击伤方法制作创伤性脑损伤模型,假手术组仅行开颅术,不造成脑损伤。醒脑静组在大鼠造模后10 min内经尾静脉注射醒脑静注射液10 ml/(kg·d),模型组与假手术组则经尾静脉注射等量0.9%氯化钠溶液,三组均连续给药7 d。给药第7天比较各组大鼠血清中S-100B蛋白和神经特异性烯醇化酶(NSE)水平,脑组织含水量,检测血清中超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)含量,并对各组大鼠进行神经功能缺损评分。结果:与假手术组比较,醒脑静组和模型组均有明显的神经缺损,脑组织含水量、MDA、S-100B蛋白和NSE水平明显升高,SOD、GSH-Px含量明显降低;醒脑静组与模型组比较,醒脑静组神经缺损程度及脑含水量显著低于模型组,血清中MDA和NSE水平明显低于模型组,SOD、GSH-Px活性明显高于模型组。结论:醒脑静注射液对大鼠颅脑损伤具有保护作用,其作用机制可能与减轻颅脑损伤后脑水肿及抑制氧自由基反应、保护神经细胞有关。

Objective： To investigate the protective effect and mechanism of Xingnaojing（Traditional Chinese Medicine） injection on brain injury in rats. Methods： Sixty-three healthy adult male SD rats were randomly divided into 3 groups （ n = 21） ： sham operation group, model group, xingnaojing group. The model of traumatic brain injury model group and Xingnaojing group used the free fall impact injury method, the sham operation group underwent cmniotomy, did not cause brain damage. Xingnaojing group in rats after 10 min by tail vein injection Xingnao- jing injection 10 ml/ （kg·d）, model group and sham operation group were intravenously injected with 0.9% sodium chloride solution, three groups were administered continuously for 7 days. At administration of the seven^th days compared the S-100B protein in the serum and neuro specific enolase （NSE） level, the water content of brain tissue, serum superoxide dismutase （SOD）, malondialdehyde （MDA） and glutathione peroxidase （GSH-Px） content, and neurological function of rats among groups. Results： Compared with the sham operation group, the nerve defect, brain water content, MDA, S-100B protein and NSE levels were obvigusly increased in Xingnaojing group and model group; SOD, GSH-Px content decreased significantly ; In Xingnaojing group nerve impairment and brain moisture were significantly lower than those of model group, the serum MDA, S- 100B protein and NSE levels were significantly lower than those in the model group, the SOD, GSH-Px activity was significantly higher than that in the model group. Conclusion： Xinguaojing injection has protective effects on rat brain injury, and its mechanism may be related to reduce brain edema after traumatic brain injury and inhibit the reaction of oxygen free radical, protect nerve ceils.