鼻息肉组织中TSLP的表达及与Th2炎症反应的关系

The relationship of the expression of thymic stromal lymphopoietin in nasal polyps tissues and Th2 inflammatory response

目的:探讨鼻息肉组织中TSLP的表达及与Th2炎症反应的关系。方法:收集60例确诊为鼻息肉的患者,采用免疫组织化学SP染色法检测其鼻息肉组织中TSLP的表达,ELISA法检测组织中IL-4、IL-5、IFN-γ及IL-13的表达,并分析其与TSLP表达的相关性。结果:TSLP在鼻息肉组织中较正常下鼻甲黏膜表达高,而IL-4、IL-5、IFN-γ和IL-13在鼻息肉组织表达显著增高(P<0.05)。TSLP与IL-4、IL-5、IL-13呈显著正相关(r分别为0.475、0.594和0.582,P<0.01),TSLP与IFN-γ呈显著负相关(r=-0.614,P<0.01)。结论:TSLP高表达可能会促进T细胞向Th2方向分化,参与鼻息肉的发生、发展,并加重鼻部Th2炎症反应。

Objective：To explore the relationship of the expression of thymic stromal lymphopoietin （TSLP） in nasal polyps tissues and the Th2 inflammatory response. Method：Sixty patients with nasal polyps were collected. The immunohistochemical staining method was used to detect the expression of TSLP in nasal polyps tissues and ELISA method to the expression of IL-4, IL-5, IFN-γ, IL-13 and analyzed the correlation between them. Result：The expression of TSLP in nasal polyp tissues was higher than that in normal inferior turbinate mucosa （P〈0.05）. The expression level of IL-4, IL-5, IFN-γ and IL-13 in nasal polyps tissues were significantly higher than those in control group （P〈0.05）. TSLP staining was a statistically significant positive correlation with IL- 4,IL-5 and IL-13（r=0. 475, 0. 594 and 0. 582, respectively, P〈0.01）, while inverse correlation with IFN-γ （r= -0.614,P〈0.01）. Conclusion： The high expression of TSLP might promote T cell differentiation towards Th2, and participated in the occurrence/development of nasal polyps, aggravated the nose Th2 inflammatory response.