大鼠白介素-1β在异氟烷预处理脊髓损伤中的作用

The effect of interleukin-1β on spinal cord injury after isoflurane preconditioning in rats

目的探讨白介素-1β（IL-1β）在异氟烷预处理大鼠脊髓损伤（spinal cord injury，SCI）时的作用。方法：雄性成年SD大鼠75只，体重250—300 g，将大鼠随机分为3组（n=25）：假手术组（S组）、脊髓损伤组（SCI组）、异氟烷预处理组（ISO组）。采用打击法制作脊髓损伤模型。ISO组吸入2％异氟烷2 h，24 h后制备脊髓损伤模型。连续7 d采用BBB评分进行神经功能缺陷评分，免疫荧光标记神经元特异性核蛋白（neuron-specific nuclear protein，NeuN）和白介素-1β的表达，其余动物处死，采用Western Blot法测定白介素-1β的表达水平。结果：与S组比较，SCI组和ISO组神经功能缺陷评分降低，NeuN和IL-1β表达增加，IL-1β蛋白表达上调（P〈0．05）；与SCI组比较，ISO组神经功能缺陷评分增高，NeuN和IL-1β表达下降，IL-1β蛋白表达下调（P〈0．05）。结论：异氟烷预处理可能通过抑制IL-1β表达，从而减轻大鼠脊髓损伤。

Objective： To explore the effect of interleukin-lbeta（ IL-1β） on spinal cord injury（ spinal cord injury, SCI） after isoflurane preconditioning in rats. Methods ： Seventy-five adult male SD rats, weighing 250 - 300 g were randomly divided into three groups （n =25） ： sham operation group （S group）, spinal cord injury group （SCI group）, isoflurane preconditioning group （ISO group）. Spinal cord injury model was made by percussion. Inhalation of 2% isoflurane for 2 h in rats of ISO group was performed to make SCI 24 h post treatment. Neurological deficit scores were evaluated by BBB score for consecutive seven days, the expression of NeuN （neuron-specific nuclear protein） and IL-1β were labeled by immunofluorescence, the rest rats were killed and the expression of IL-1β protein was detected by Western Blot. Results： Compared with S group, the neurological deficit score was significantly lower. The expression of NeuN and IL-1β was increased and IL-1β protein was up-regulated in SCI and ISO group（ P 〈 0.05 ）. Compared with SCI group,the neurological deficit score was significantly enhanced, the expression of NueN and IL-1β was reduced, IL-1β protein was down-regulated in ISO group （ P 〈 0.05 ）. Conclusion ： Isoflurane preconditioning could reduce spinal cord injury by inhibition of IL- 1β.