肝癌衍生生长因子-2在大鼠脊髓损伤后的表达及其意义

Expression change and significance of hepatoma-derived growth factor-2 in rat injured spinal cord

目的:探讨肝癌衍生生长因子(HDGF)-2在大鼠脊髓损伤后的表达及其意义。方法:取成年SD大鼠120只,随机分成正常对照组和脊髓损伤组。采用改良Allen’s法建立脊髓损伤动物模型,分别于损伤后1、21、45d分别采集受损伤的脊髓标本。应用免疫组织化学染色技术观察HDGF-2在正常脊髓、受损伤后不同时间点的脊髓组织中的表达情况。结果:(1)HDGF-2在大鼠正常脊髓和受损伤脊髓前角神经元和胶质细胞的细胞核中均有表达。(2)脊髓神经细胞核内HDGF-2染色评分,在正常组为50±9,脊髓损伤组为132±30,两组间HDGF-2表达比较具有显著性差异(P<0.01)。(3)HDGF-2表达评分在术后1、21、45 d分别为141±62,107±33和92±18,脊髓损伤不同时间点HDGF-2表达评分比较具有显著性差异(P<0.01)。结论:脊髓损伤后HDGF-2表达升高,提示其参与脊髓损伤和修复的病理过程。

Objective： To explore expression change and significance of hepatoma-derived growth factor-2 （HDGF-2） in rat after spinal cord injury （SCI）. Methods： 120 adult Spragne-Dawley （SD） rats were randomly divided into normal control and spinal cord injury group. In spinal cord injury group, adult rats were administered contusion SCI, at the TIO vertebrae level, with a modefied Allen＇s impactor. The spinal cord was carefully dissected at 1,21 and 45 days post operation. The expressions of HDGF-2 was detected by immunohistochemical staining. Results ： （ 1 ） HDGF-2 expression was primarily localized in the nuclei of neurons, astrocytes and oligodendrocytes in the anterior horn. Intense HDGF-2 was seen in the spinal cord lesion area. （2） Scores of HDGF-2 expression were respectively 50 ± 9 in normal spinal cord, and 132 ± 30 in injured spinal cord. There were significant difference between normal and injured spinal cord （P 〈 0.01 ）. （3）Intense HDGF-2 scores were respectively 141 ±62 at 1 day, 107 ±33 at 21 days and 92 ± 18 at 45 days post-operation. Expressions of HDGF-2 in spinal cord tissues after SCI were significantly different among different post-operative stages （P 〈0. 01 ）. Conclusions： After spinal cord injury, the expression of HDGF-2 increases, and it is involved in the pathological process of spinal cord injury and repair.