不同浓度MPA对Ishikawa细胞PARP-1表达的影响

PARP- 1 expression of Ishikawa cells treated with different concentration of MPA

目的:研究不同浓度孕激素作用子宫内膜癌Ishikawa细胞后DNA损伤修复蛋白PARP-1的表达变化,探讨PARP-1在子宫内膜腺癌中的作用及PARP-1和孕激素相互关系。方法:免疫组化SP法和实时荧光定量PCR(Real-time PCR)法检测不同浓度的醋酸甲羟孕酮(Medroxyprogesterone acetate,MPA)干预Ishikawa细胞后PARP-1蛋白及PARP-1 mRNA表达的变化。结果:免疫组化显示PARP-1蛋白表达于Ishikawa细胞核,随着MPA浓度的增加PARP-1的表达呈下降趋势。RT-PCR检测到MPA呈浓度依赖性地抑制PARP-1 mRNA表达。结论:醋酸甲羟孕酮呈浓度依赖性地抑制PARP-1的表达,提示孕激素可能间接或直接通过抑制PARP-1而发挥治疗子宫内膜癌的作用。

Objective：To study the effect of PARP-1 expression of Ishikawa cells treated with different concen-tration of MPA,discussed the role of PARP-1 in the development of endometrial cancer,and the mutual relations be-tween PARP-1 and progesterone. Methods：The expression of PARP-1 was detected by immunohistochemical tech-nique,and PARP-1 mRNA by real-time PCR. Results：Immunohistochemistry showed that PARP-1 protein ex-pression in Ishikawa cell nucleus,with the increase of the concentration of MPA PARP-1 expression showed a down-ward trend. RT-PCR detected MPA concentration -dependent inhibition of PARP -1mRNA expression. Conclu-sion：Medroxyprogesterone acetate can affect DNA repair protein PARP-1 expression,to some extent,inhibited the expression of PARP-1 showed a concentration dependent,and also suggest progesterone may directly or indirectly by inhibiting PARP-1 play a role in the treatment of endometrial cancer.