Bcl-2、p53和ki-67在甲状腺癌中的表达及其临床意义的研究

Bcl- 2,p53 and ki- 67 expression in thyroid carcinoma and its clinical significance

目的:研究甲状腺癌组织中Bcl-2、p53和ki-67的表达及其临床意义。方法:采用免疫组织化学SP法检测甲状腺癌组织、甲状腺腺瘤和正常甲状腺中Bcl-2、p53和ki-67的表达情况。结果:Bcl-2、p53和ki-67的阳性表达见于甲状腺癌组织和甲状腺腺瘤中,正常甲状腺组织中未见三者的阳性表达。甲状腺癌组织中Bcl-2的阳性表达率明显高于腺瘤组织(P<0.05),甲状腺癌组织中p53的阳性表达率明显高于腺瘤组织(P<0.05),ki-67在甲状腺癌中的阳性表达率为71.3%,高于甲状腺腺瘤10.0%(P<0.05)。甲状腺未分化癌和滤泡状癌组织中Bcl-2的阳性率明显增高。有淋巴结转移和临床Ⅲ、Ⅳ期病例中,Bcl-2的阳性率明显增高。结论:Bcl-2、p53和ki-67在甲状腺癌发生发展中起重要作用,Bcl-2、p53和ki-67的表达可作为甲状腺癌预后的指标。

Objective：To investigate expressions of Bcl-2,p53 and ki-67 in thyroid carcinoma and their clinical significance. Methods：SP immunohistochemical method was used to assess the expressions Bcl-2,p53 and ki-67 in thyroid carcinoma（ TC group）,thyroid adenoma（ TA group）and normal thyroid tissues（ control group）. Results：Bcl-2 was expressed in 57. 5% of the thyroid carcinoma,20. 0% of the thyroid adenoma,and non in normal thyroid tissues. The positive expression rate of Bcl-2 in the thyroid carcinoma was significantly higher than that in the thyroid adenoma（P〈0. 05）. The positive expression rate of p53 in the thyroid carcinoma was significantly higher than that in the thyroid adenoma（P〈0. 05）. The positive expression rate of ki-67 in the thyroid carcinoma was significantly higher than that in the thyroid adenoma（P〈0. 05）. In thyroid carcinoma,undifferentiated carcinoma,follicular carci-noma,carcinoma with positive lymph nodes,and tumors with stage Ⅲ andⅣ disease had significantly higher expres-sion of Bcl-2. Conclusion：Bcl-2,p53 and ki-67 play an important role in the development of thyroid carcinoma and the overexpression of them might be a possible prognostic factor of thyroid cancer.