摘要
目的:探讨核酸定量检测在HIV-1感染实验室诊断中的应用。方法:选取145例第四代抗原/抗体联合诊断筛查试验为阳性反应的血浆样本,分别用Western印迹和HIV-1核酸定量方法进行检测,综合对比分析2种方法检测结果。结果:Western印迹检出阳性样本120例,不确定样本17例,阴性样本8例;HIV-1核酸定量试验检出结果大于检测限样本131例,其中包括12例Western印迹不确定样本、2例Western印迹阴性样本;有3例Western印迹阳性样本用HIV-1核酸定量检测试验未能检出。结论:核酸定量检测试验对于HIV-1感染阳性样本是一种有效的实验室诊断方法;对HIV-1核酸定量检测结果为"TND"的样本,建议加做Western印迹或结合其他补充试验结果进行综合诊断。
Objective： To study the performance of the quantitative HIV RNA detection for HIV-1 diagnosis. Methods： A total of 145 plasmas that were reactive on fourth-generation screening assays were tested by Western blotting（WB） and HIV-1 RNA quantitative assay. The results of two assays were analyzed by synthesis. Results： 120 out of 145 samples were confirmed to be HIV positive by WB, 17 samples were classified as uncertain and 8 were HIV negative. 131 samples were detected by HIV-1 RNA including 12 indeterminate HIV antibody sam- ples and two samples tested negative by WB. Three samples confirmed by WB were not detected by HIV-1 RNA. Conclusion： In this study, HIV-1 RNA quantitative asssy is a efficient methods to discriminate the positive results. Samples that are not detected by HIV-1 RNA would required WB or other tests.
出处
《生物技术通讯》
CAS
2014年第3期385-387,共3页
Letters in Biotechnology
基金
国家科技重大专项(2012ZX10001001-001-002)
