蜻蜓凤梨FT1基因克隆与植物表达载体构建研究

Construction of Plant Expression Vector of FT1 Gene from Aechmea fasciata

为了研究凤梨科植物开花的机理,本研究利用5'RACE和3'RACE方法从蜻蜓凤梨中克隆得到响应开花的主要调控基因FT1。该基因CDS序列全长为625 bp。为了进一步研究其功能,本研究将其重组并构建了以CaMV35S为启动子、GUS为报告基因的植物表达载体CaMV35S-GUS-FT1,并采用农杆菌介导的方法转化云烟K346烟草的幼嫩叶片,经GUS组织化学染色观察转基因烟草体细胞中GUS报告基因的表达情况。研究结果验证了重组构建的FT1基因载体的正确性,为进一步解析FT1基因在蜻蜓凤梨开花过程中的功能奠定了基础。

In order to investigate the mechanism ofbromeliads, we cloned Flowering Locus 1 （FiI）, a key gene response to flowering using 5＇RACE and 3＇RACE methods from Aechrneafasciata. The full length of FT1 CDS is 625 bp. After that, we constructed CaMV35S-GUS-FT1 and transferred it into tobaccos by Agrobacterium-mediated transformation method. Using GUS histochemical staining we found the FT1 gene was successfully expressed in transgenic plants. Our results may lay foundation for the further studying of the function and application of FT1 gene.