珍稀濒危植物太行菊遗传多样性的SRAP分析

Genetic Diversity of the Rare and Endangered Plant Opisthopapus taihangensis (Ling) Shih Deteced by SRAP Analysis

本研究利用SRAP分子标记技术对太行山特有濒危物种太行菊11个自然居群的遗传多样性进行研究。采用10对引物对11个居群的151个样品进行扩增,共得到90个扩增位点,其中多态性位点73个,多态位点百分率(PPL)为81.11%。POPGENE分析显示,太行菊具有较高的遗传多样性(H=0.205 9,I=0.325 6)。Nei's遗传多样性分析表明,11个自然居群间出现了较高的遗传分化(基因分化系数Gst=0.274 9,基因流Nm=1.318 8)。生境的片段化和基因流障碍可能是导致太行菊居群间遗传分化显著的主要原因。通过对太行菊居群遗传多样性的研究,可为有效保护太行菊提供理论依据。

Using analysis of Sequence-related ampllified polymorphism （SRAP）, the genetic diversity of 11 natural populations of Opisthopapus taihangensis （Ling） Shih in Taihang mountain was investigated. Ten pairs of SRAP primers were used to amplify 151 samples of 11 natural populations, in this case, 90 DNA fragments were produced in total, and among them 73 bands were polymorphic loci （PPL=81.11%）. The result of POPGENE analysis indicated that genetic diversity of Opisthopapus taihangensis （Ling） Shih was higher than others （I=0.214 9, H=0.345 5）. A high level of genetic differentiation among 11 populations was detected based on Nei＇s genetic diversity analysis （Gst=0.274 9, Nm=1.318 8）. The main factors responsible for the high degree of genetic differentiation among populations may result from habitat fragmentation and barriers of gene flow. The study on genetic diversity of Opisthopapus taihangensis （Ling） Shih will provide theoretical basis for conservation of them.