竹子叶绿体基因组SSR分子标记的开发及其应用

Development and Application of SSR Molecular Markers from the Chloroplast Genome of Bamboo

为探讨观赏竹叶片异质性的机理,根据麻竹(Dendrocalamus latiflorus)和绿竹(Bambusa oldhamii)叶绿体基因组序列开发SSR分子标记。结果表明,在麻竹和绿竹叶绿体基因组中分别存在87和86个SSR位点,其中三核苷酸重复类型最多,其次为单核苷酸重复类型。根据SSR位点设计21对引物,其中11对引物对6竹种能够扩增出稳定、清晰的条带,且具有多态性,引物有效率达到52.4%。聚类分析表明,6竹种可分为两大类群,与形态学分类结果基本一致。有4对引物在菲白竹(Pleioblastus fortunei)和白纹椎谷笹(Sasaella glabra f.albo-striata)的花叶中具有多态性,可作为区分观赏竹叶片异质性的分子标记。

In order to reveal the heterogeneity mechanism of ornamental bamboo leaves, the SSR molecular markers were developed from chloroplast genome of Dendrocalamus latiflorus and Bambusa oldhamii. The results showed that there were 87 and 86 SSR loci in chloroplast genome of D. latiflorus and B. oldhamii, respectively, in which the trinucleotide type was the most, followed by mononucleotide type. Based on the sequences of SSR loci in chloroplast genome, 21 pairs of SSR primers were designed. Six bamboo species could be ampliifed stable and clear bands with polymorphism by 11 pairs of primers, accounting for 52.4%. The cluster analysis indicated that 6 bamboo species could be divided into two groups, which was consisted with morphological classiifcation. Meanwhile, polymorphism bands could be ampliifed in different parts of colorful leaves from Pleioblastus fortunei and Sasaella glabra f. albo-striata by 4 pairs of primers. So, it was suggested that these SSR markers could be used to distinguish the leaf heterogeneity of ornamental bamboo.