摘要
目的建立红景天苷在大鼠血浆内的UPLC-MS/MS检测方法,研究红景天苷在大鼠体内的生物利用度。方法以茶碱为内标,运用UPLC-MS/MS法检测大鼠灌胃(20 mg/kg)和尾静脉注射(5 mg/kg)给药后血浆中红景天苷的血药浓度,应用DAS2.1.1药动学软件对数据进行分析。结果主要药动学参数:口服红景天苷在大鼠血浆中的消除半衰期(t1/2 z)为(49.56±19.26)min;最高血药浓度(Cmax)为(3.28±0.66)mg/L;平均滞留时间(MRT0-t)为(56.84±6.61)min;曲线下面积(AUC0-∞)为(235.95±65.72)mg/(L·min);清除率(CL)为(0.091±0.027)L/(min·kg);经剂量校正后,口服的绝对生物利用度为33.17%。结论该方法灵敏度高,专属性强,适用于红景天苷在大鼠血浆中血药浓度的测定。
AIM To establish a UPLC-MS/MS method to determine salidroside in the rat plasma, and to calculate the pharmacokinetic parameters of salidroside in rats after oral administration. METHODS Salidroside was administered to the rats （5mg/kg body weight） by intravenous injection or by （20mg/kg body weight） oral gara- ges. Theophylline was taken as the internal standard. DAS2. 1.1 was used to process the pharmacokinetic data. RESULTS The pharmacokinetic parameters of t1/2z, Cmax , MRT0-1, AUC0-∞ and CL were as follows ： （49.56±19.26） min, （3.28 ±0. 66） mg/L, （56. 84±6. 61 ） rain, （235.95±65.72） mg/L ·min, （0. 091 ±0. 027） L/ min·kg. After caliberation, the bioavailability of salidroside in rats was 33.17% . CONCLUSION An accurate LC-MS/MS method with high sensitivity and specificity for determining salidroside has been developed and success- fully applied to the pharmacokinetic study of salidroside in rats.
出处
《中成药》
CAS
CSCD
北大核心
2014年第6期1176-1181,共6页
Chinese Traditional Patent Medicine
基金
江苏省优势学科支持计划(YSXK-2010)
