HPLC法同时测定妇炎净片中4种成分

Simultaneous determination of four constituents in Fuyanjing Tablets by HPLC

目的建立同时测定妇炎净片（苦玄参、当归、五指毛桃等）中苦玄参苷IA、阿魏酸、补骨脂素和异补骨脂素4种成分的高效液相色谱测定方法。方法采用KromasilC18色谱柱（5μm，4．6mm×250mm），以1％冰乙酸-乙腈为流动相进行梯度洗脱，体积流量1．0mL／min，检测波长245nm。结果苦玄参苷IA、阿魏酸、补骨脂素和异补骨脂素分别在15～150μg/mL、2．5—25μg／mL、1．0～10μg／mL、1．2～12μg/mL范围内线性良好，r值均大于0．999，平均加样回收率分别为99．3％、98．8％、99．0％和99．2％，相对标准偏差（RSD）均小于0．7％（n=9）。结论该方法简便，结果准确，可有效地用于妇炎净片的质量控制。

AIM To establish HPLC method for determining four constituents （picfeharraenin IA, ferulic acid, psoralen and isopsoralen ） in Fuyanjing Tablets （Picriae Herba, Angelicae sinensis Radix, Fici simplicissmae Radix, etc. ）. METHODS HPLC was performed on Kromasil C18 （5 μm, 4. 6 mm ×250 mm） column with the mobile phase of 1% acetic acid solution as phase A and acetonitrile as phase B in gradient elution manner. The de- tection wavelength was set at 245 nm. RESULTS The calibration curve was linear over the concentration range of 15 - 150μg/mL for picfeltarraenin IA, 2. 5 - 25μg/mL for ferulic acid, and 1.0 ～10 μg/mL for psoralen, and 1.2 - 12 μg/mL for isopsoralen , respectively. The correlation coefficient of all curves was more than 0. 999. The average recoveries of four constituents were 99.3% for picfeltarraenin IA, 98.8% for ferulic acid, 99.0% for psoralen, and 99.2% for isopsoralen, and the RSD value of precision was less than 0. 7% （ n = 9）, respectively. CONCLUSION The developed HPLC method could be used for the quality control of Fuyanjing Tablets.