紫背金盘中痢止蒿甲素、β-蜕皮激素、8-乙酰基哈帕苷的测定

Determination of ajuforrestin A,ecdysterone and 8-O-acetylharpagide in Ajuga nipponensis Makino by HPLC

目的建立HPLC色谱法测定紫背金盘中痢止蒿甲素、β-蜕皮激素和8-乙酰基哈帕苷的量。方法采用Sino—ChromODS—BP色谱柱（250mm×4．6mm，5μm），柱温为25℃，体积流量0．8mL/min。痢止蒿甲素检测波长为228nm，流动相为乙腈-水（70：30）；β-蜕皮激素检测波长为243nm，流动相为乙腈-水（18：82）；8-乙酰基哈帕苷检测波长为212nm，流动相为乙腈-水（13：87）。结果痢止蒿甲素、β-蜕皮激素、8-乙酰基哈帕苷分别在0．24～2．40μg（r=0．9984）、0．40～4．00μg（r=0．9962）、0．40～4．00μg（r=0．9986）范围内与峰面积呈良好的线性关系，平均回收率分别为98．90％、97．36％、99．18％，RSD依次为1．74％、1．83％、1．11％。结论紫背金盘中8-乙酰基哈帕苷的含有量较高。

AIM To establish an HPLC method for determining contents of ajuforrestin A, ecdysterone and 8- O-acetylharpagide in Ajuga nipponensis Makino. METHODS The Sino Chrom ODS-BP column （250 mm ×4.6 mm, 5 μm） was used at 25℃ with the flow rate of 0. 8 mL/min. Ajuforrestin A was detected at detection wavelength of 228 nm with the mobile phase of acetonitrile-water （70 ： 30 ）. Ecdysterone was detected at detection wavelength of 243 nm with the mobile phase of acetonitrile-water （18 ： 82）. 8-O-Acetylharpagide was detected at detection wavelength of 212 nm with the mobile phase of acetonitrile-water （ 13 ： 87 ）. RESULTS The linear response ranged from 0. 24 - 2.40μg for ajuforrestin A （ r = 0. 998 4 ） , 0. 40 - 4. 00 p.g for ecdysterone （ r = 0. 996 2）, 0. 40 - 4. 00 μg for 8-O-acetylharpagide （r = 0. 998 6）, respectively. Recoveries were 98.90% with RSD 1.74% for ajuforrestin A, 97.36% with RSD 1.83% for ecdysterone, 99. 18% with RSD 1.11% for 8-0- acetylharpagide, respectively. CONCLUSION The content of 8-O-acetylharpagide in Ajuga nipponensis Makino is the highest of the three constituents.