乳腺不典型增生癌变过程中基底膜的变化

CHANGES OF BASEMENT MEMBRANE DURING CARCINOGENIC CHANGE OF THE ATYPICAL HYPERPLASIA OF THE MAMMARY

目的 观察乳腺良性疾病不典型增生癌变过程中基底膜的变化。方法 采用SP免疫组化染色法和两种特殊染色方法 (Foot及PAS染色法 ) ,并利用电镜观察基底膜的变化。结果 Ⅱ级不典型增生的乳腺组织已有基底膜的变化 ,Ⅲ级不典型增生时基底膜的变化更明显 ,主要表现为光镜下基底膜明显厚薄不均 ,电镜下则可见小的缺失 ,而乳腺癌组织中基底膜完全缺如。结论 在乳腺上皮细胞癌变过程中基底膜的变化与乳腺上皮细胞的变化密切相关 ,是乳腺上皮不典型增生癌变过程的一部分。

Objective\ To observe the changes of basement membrane (BM) during carcinogenic change of the atypical hyperplasia of the mammary. Methods\ SP immunohistochemical method and two special stain method (Foot and PAS stain method) and the electron microscope were used to observe the changes of the BM. Results\ BM already changed in atypical hyperplasia grade Ⅱ, and more significantly changed in atypical hyperplasia grade Ⅲ. BM was thinner or thicker somewhere distinctively under the microscope, and some little deletions were observed under the electron microscope in atypical hyperplasia grade Ⅲ, and there was no BM in breast cancer. Conclusion\ The change procedure of BM was correlated with the changes of the epithelial, atypical hyperplasia. The changes of BM in the atypical hyperplasia are a part of the carcinogenic procedure from the epithelial atypical hyperplasia of the mammary.