针刺对离心运动大鼠骨骼肌生肌调节因子的影响

Effects of Acupuncture on Skeletal MRFs in Rats after Eccentric Exercise

目的:观察4周离心运动及针刺干预对生肌调节因子(MRFs)的影响,探讨MRFs变化特征及针刺在骨骼肌损伤修复中的作用。方法:72只雄性SD大鼠分为安静组(C)、运动组(E)和运动针刺组(EA),E与EA组进行坡度为-16°的4周跑台运动;免疫印迹法检测生肌调节因子MyoD、Myf5、Myogenin、MRF4蛋白表达。结果:第1、3、4周E组MyoD、MRF4蛋白表达显著低于C组和EA组(P<0.05);EA组MyoD蛋白表达第1、3、4周接近安静水平,MRF4蛋白表达第4周显著低于C组(P<0.05)。E组Myf5蛋白表达呈上升趋势,第4周显著高于C组(P<0.05);EA组Myf5蛋白表达第1至3周均高于E与C组;E组Myogenin蛋白表达持续升高,且第3、4周显著高于C组(P<0.05);EA组Myogenin蛋白表达第1、2周显著高于E组(P<0.05),第3、4周与之相反。结论:4周离心运动能上调Myf5与Myogenin蛋白表达,下调MyoD与MRF4蛋白表达;针刺具有调控骨骼肌MRFs保持正常水平的作用。

Objective The aim of this study is to observe the effects of four-week eccentric exercise and acupuncture on the myogenic regulatory factors（MRFs）. Methods 72 male SD rats were divided into three groups：control group （C）,exercise group （E）and exercise plus acupuncture group （EA）. Rats in groups E and EA were submitted to four-week downhill running （-16°）on a treadmill for 4 weeks. Rats in groups EA received acupuncture therapy immediately after running. Protein expression of MyoD, MyfS,myogenin and MRF4 were detected by western blot. Results The MyoD and MRF4 protein in group E decreased significantly comparing to groups C and EA in the lst,3rd,and 4th week（P 〈 0.05）. MyoD protein in group EA was close to normal level in the lst,3rd,and 4th week,and MRF4 protein significantly lower than control group in the 4th week （P 〈 0.05）. Myf5 protein in group EA was higher than groups E and C from the 1st to 3rd week. Myogenin protein in group E went up steadily and was significantly higher than in group C in the 3rd and 4th week （P 〈 0.05）. Myogenin protein in group EA was higher than in group E in the 1st and 2nd week（P 〈 0.05） ,but was inversely lower than in group E in the 3rd and 4th week （P 〈 0.05）. Conclusion Four-week eccentric exercise may up-regulate Myf5 and myogenin protein expression,and down-regulate MyoD and MRF4 protein expression,meanwhile acupuncture can maintain normal levels of MRFs.