脑胶质瘤抑制因子Glob-N分子结构的初步鉴定

PRELIMINARY STUDY OF THE MOLECULAR STRUCTURE OF GLOB-N:A GLIOMA INHIBITOR

为分析脑胶质瘤抑制因子N 红细胞糖苷脂 (Glob N)的分子结构 ,运用核磁共振仪及质谱仪分析了人O型血红细胞膜、牛脑及人脑Glob N的结构。结果显示 ,红细胞Glob N为四糖基神经酰胺 ,牛脑与人脑Glob N均为三糖基神经酰胺 ,3种Glob N中神经酰胺所含的碳原子数目不同 ,分别为 2 7、33和 2 6个。说明Glob N为一类中性鞘糖脂 ,其结构与组织来源相关。

In order to study the molecular structure of Glob-N, a glioma inhibitor, Glob-N was separated from the membrane of human type O red blood cells, cow brain and normal human brain tissue respectively. Their structures were identified by using time-of-flight mass spectrometry, 1H-NMR and 13C-NMR. The results showed that the structure of Glob-N from the membrane of human type O red blood cells was proposed as globotetraosylceramide, while Glob-N from cow brain and normal human brain was globotriaoceramide, and the number of carbon atoms in the ceramide of the Glob-N was 27, 33 and 26 respectively. It suggested that Glob-N is a kind of neutral glycosphingolipids, and its molecular structure is different in different tissues.