卵巢癌细胞株COC1/DDP多药耐药逆转对中性鞘糖脂表达的调控作用

REGULATION OF EXPRESSION OF N-GSLS AND THE REVERSION OF MDR IN OVARIAN CANCER CELLS (COC1/DDP)

为探讨逆转卵巢癌细胞株的多药耐药性 (MDR)对细胞表面中性鞘糖脂 (N GSLs)表达的调控作用 ,以三苯氧胺 (TAM )和维拉帕米 (VRP)为逆转剂 ,用MTT法分析了TAM、VRP的逆转效应 ,采用改良的Hakamori方法提取、纯化了耐药亚株COC1/DDPMDR逆转前后及亲本株COC1细胞的N GSLs,高效薄层层析(HPTLC)分析了N GSLs的表达。结果显示 ,COC1/DDP和COC1的N GSLs表达有显著差异 ,COC1/DDP的CMH表达较COC1强 ;经TAM、VRP作用后 ,COC1/DDP的耐药性发生逆转 ,CMH表达降低。说明卵巢癌的多药耐药性与中性鞘糖脂CMH相关 ,CMH可能为卵巢癌MDR相关鞘糖脂抗原。

In order to evaluate the relationship between MDR of ovarian cancer and expression of neutral glycosphingolipids(N-GSLs) in ovarian cancer cell lines, the effects of TAM and VRP on the growth of COC1/DDP were assayed by MTT method. N-GSLs of the cells were isolated and purified with the modified Hakamoris method and analysed by HPTLC. The results showed that the expression of N-GSLs was different between parent cell lines and resistant cell sublines, the level of CMH was higher in COC1/DDP than in COC1. TAM and VRP could render multidrug-resistant cells sensitive to chemotherapy, while the level of CMH concomitantly was sharply decreased. It suggested that the expression of N-GSLs is associated with MDR of ovarian cancer, and CMH may be a kind of MDR related glycolipids in ovarian cancer.