信号转导机制在大鼠脑损伤后血脑屏障损害中的作用

THE EFFECT OF SIGNAL TRANSDUCTION MECHANISM ON BBB DAMAGE IN BRAIN INJURY OF RATS

为探讨信号转导系统在脑损伤后血脑屏障损害中的作用 ,取雄性SD大鼠 75只 ,随机分为正常对照组、假手术组、脑损伤组、毛喉素组和H7组 ,用荧光显微镜定性观察、分光光度计定量检测伊文蓝渗出。结果显示 ,脑损伤后伊文蓝明显渗出 ,毛喉素组和H7组渗出明显减少 ,提示毛喉素和H7能阻止血脑屏障开放 ,可望为血脑屏障保护提供新的治疗途径。

This study was designed to explore the mechanism of signal transduction on BBB damage in brain injury. 75 male SD rats were randomly devided into 5 groups: control, sham operation, injury only, injury with Forskolin-treatment and H7 treatment.Evans blue permeation was observed qualitatively with an epifluo resence and mesured quantitatively with a spetrophotometer.The result shcwed that Evans blue significantly permeated in injuried group,and noticeably decereased in H7 treatment group and Forskolin treatment group. It indicated that Forskolin and H7 can effectively prevent BBB opening,and suggest that Forskoling and H7 may provide new ways on BBB protection.