乳腺癌血管生成与预后的相关性

STUDY OF THE CORRELATION BETWEEN ANGIOGENESIS AND PROGNOSIS OF BREAST CARCINOMA

随访 92例原发性乳腺癌术后病例。在石蜡包埋肿瘤组织中 ,以免疫组化方法检测血管内皮生长因子 (VEGF)表达和肿瘤内微血管密度 (MVD) ,并对二者进行相对定量观察。结果无瘤生存 (RFS)时间单变量分析显示 ,在淋巴结阴性组中 ,高MVD组比低MVD组的RFS时间降低 ,差异有显著性意义 (P <0 .0 5 )。RFS多变量分析表明 ,在淋巴结阴性组和全部病例组中 ,MVD是一个独立的预后因素 (P <0 .0 5 0 .0 1)。此外 ,VEGF表达程度与MVD之间有显著的正相关关系 (P <0 .0 1)。结果显示 ,VEGF在原发性乳腺癌血管生成中起着关键作用 ;VEGF和MVD与乳腺癌的生物学行为关系密切 。

92 specimens from patients with invasive ductal carcinoma were investigated. The expression of vascular endothelial growth factor (VEGF) was demonstrated by immunocytochemical staining with an antibody against VEGF and was quantitatively estimated by using computerized image analysis system. Vessels were immunohistochemically highlighted by using an antibody to CD34, and microvessel density (MVD) was quantified. The postoperative survey in univariate analysis showed that the relapse-free-survival (RFS) time of patients with more than 87 microvessels in single microscopic field was significantly worse compared to that of patients with less than 87 microvessels in node-negative patients (P<0.05). Multivariate analysis confirmed that MVD was an independent prognostic indicator for RFS in node-negative patients and all patients (P<0.05~0.01). In addition, there was a closely positive correlation between VEGF and MVD (P<0.01). The results suggested that VEGF plays crucial roles in the promotion of angiogenesis in primary breast carcinoma. VEGF and MVD are closely correlated with biological behavior of primary breast carcinomas. MVD can provide a useful message in predicting the recurrence or metastasis of tumors.