菌斑pH转化试验与菌斑中变形链球菌感染水平的相关性研究

Assessment of the Caries Activity Test (Cariostat) Based on the Infection Levels of Mutans Streptococci in 3 to 14-year-old Children's Dental Plaque.

目的 探讨菌斑 pH转化试验 (Cariostat)与菌斑中变形链球菌感染水平之间的相关性。 方法 对 6 0名儿童 (乳牙列组儿童 35名 ,混合牙列组儿童 2 5名 )分别取左、右上后牙颊面牙菌斑 ,用右侧上后牙菌斑做Cariostat试验给出龋活性得分。同时将左侧上后牙颊面菌斑涂于TYCSB培养基 ,厌氧培养 48小时后计数菌落数。采用Spearman相关分析进行统计。 结果 乳牙列组儿童和混合牙列组儿童菌斑pH转化试验与菌斑中变形链球菌感染水平相关系数分别为r =0 .412 (P <0 .0 5 )和r =0 .6 5 1(P <0 .0 1)。结论 菌斑pH转化试验与菌斑中变形链球菌感染水平呈正相关。

Objective To explore the correlation between the Cariostat and infection levels of mutans Streptococci in children's dental plaque. Methods 60 children participating in the study were divided into two groups. The first group consisted of 35 children with primary dentition and the second group consisted of 25 children with mixed dentition. The plaque samples collected from 54, 55 or 14, 15 buccal surfaces were immediately placed into an ampule containing 2ml of Cariostat test medium which was incubated at 37℃ for 48h and Cariostat score was recorded. The plaque samples collected from 64, 65 or 24, 25 buccal surfaces were cultured on TYCSB at 37℃ for 48h and the CFU of the mutans Streptococci were counted. Spearman rank correlation coefficient was used to assess the results. Result The correlation coefficient between Cariostat score and infection levels of mutans Streptococci in the primary dentition and mixed dentition were 0.412(P<0.05) and 0.651(P<0.01) respectively. Conclusion The Cariostat and the infection levels of mutans Streptococci have significant positive correlations.