液态型凝乳酶储藏条件的初步研究

Investigation on preservation of milk-clotting enzyme

为进一步延长液态型凝乳酶的储藏时间,以金属离子、糖类、多羟基醇、大分子助剂和表面活性剂等为稳定剂优化得到最优的复合保护剂配方:山梨醇7.5%、明胶1%、甘油15%、吐温400.25%、氯化钾50 mmol/L;添加0.1 g/L对羟基苯甲酸乙酯可有效抑制在储藏过程中由杂茵繁殖导致的发臭、浑浊。添加复合保护剂和防腐剂的液态型凝乳酶,室温放置90 d,凝乳酶活力保留率为80.8%,比对照组提高41.6%。

In order to extend the preservation of milk-clotting enzyme （ MCE）, the effects of protective agents such as salts, surfactants, polyols and sugars, etc. on the MCE stability were investigated. The results showed that the optimal composite protective agents were as follows： sorbitol 7.5%, gelatin 1%, glycerol 5%, Tween 40 0.25%, KC1 50 mmol/L. The addition of 0. 1 g/L ethylparaben could effectively reduce turbidity and smell. The residual activity maintained 80.8% after stored at room temperature for 90 days.