乳腺不典型增生组织中Hras基因突变的检测

Detection of H-ras gene mutations in human breast atypical hyperplasia

目的 :探讨H ras基因在乳腺癌发生早期的作用。方法 :用PCR RFLP和PCR SSCP方法检测 30例乳腺癌、36例单纯性增生、31例不典型增生组织中H ras基因第 12密码子的突变 ,用免疫组化技术检测乳腺癌和乳腺增生病组织中H ras蛋白的表达。结果 :73 3%的乳腺癌和 48 4%的乳腺导管不典型增生组织有H ras蛋白表达 ,单纯性增生上皮中无表达 ,所检测的增生病和乳腺癌组织中均未见到H ras基因第 12密码子突变。结论 :H ras蛋白过表达出现于乳腺癌发生的早期阶段 ,但这种过表达与H ras基因第 12密码子突变无关。

Purpose To investigate the role of H ras oncogene in the early stages of human breast carcinogenesis. Methods Thirty cases of human breast cancer, 36 epithelial hyperplasia of usual type and 31 atypical hyperplasia were employed to detect H ras gene codon 12 mutations by PCR RFLP and PCR SSCP assays, and to detect the expression of H ras protein by immunohistochemistry method. Results Expression of H ras protein were found in 73 3%(22/30) of breast cancer and 48 4%(15/31) of atypical hyperplasia. No H ras protein expression was observed in hyperplasia of usual type. All tested samples of breast cancer and hyperplsia showed no mutations of H ras gene codon 12. Conclusion Overexpression of H ras protein is involved in early stages of breast carcinogenesis, but mutations of H ras gene codon 12 is rarely present in the stage.