区域性动脉灌注化疗对胰腺癌bcl-2、bax基因的影响

Effects of regional intra-arterial chemotherapy on bcl-2 and bax expression in pancreatic cancers

目的 探讨区域性动脉灌注化疗对胰腺癌bcl 2、bax基因表达的影响 ,寻找胰腺癌治疗的新手段。方法 应用TUNEL法检测 3 2例未经区域性动脉灌注化疗和 19例经区域性动脉灌注化疗的胰腺癌细胞凋亡指数 (AI) ;并采用免疫组织化学方法检测区域性动脉灌注化疗对胰腺癌细胞bcl、bax基因表达的影响。结果 区域性动脉灌注化疗组胰腺癌细胞AI比未区域性动脉灌注化疗组明显升高 ( 0 65 %vs 1 10 % ,P <0 0 5 ) ;与未区域性动脉灌注化疗组相比 ,区域性动脉灌注化疗组肿瘤细胞bcl 2基因表达明显减少 ( 3 6 7%vs 75 0 % ,P <0 0 5 ) ,而bax基因的表达显著增加 ( 73 6%vs3 4 0 % ,P <0 0 5 )。结论 区域性动脉灌注化疗可以诱导胰腺癌肿瘤细胞凋亡的增加 ,是胰腺癌综合治疗的有效措施之一。

Objective To investigate the effects of intra-arterial chemotherapy on Bcl 2 and Bax expression in pancreatic cancers to seek new approaches for treatment of pancreatic cancer. Methods Apoptotic index (AI) was detected by terminal-deoxynucleotide transferase mediated d-UTP nick end labeling (TUNEL) and expression of Bcl-2 and Bax with immunohistochemistry in 10 cases of pancreatic cancer treated by intra-arterial chemotherapy (group A) and 32 cases without the treatment (group B). Results AI was significantly higher in group A than in group B (P<0.05). The expression of Bcl-2 was markedly decreased but that of Bax remarkably increased in group A as compared with group B (P<0.05). Conclusions Intra-arterial chemotherapy can upregulate apoptosis of pancreatic cancer cells.