HPLC同时测定抱茎獐牙菜中獐牙菜苦苷、龙胆苦苷和芒果苷含量

Simultaneous Determination of Swertiamarin,Gentiopicroside and Mangiferin in Swertia franchetiana by HPLC

目的：建立抱茎獐牙菜质量标准中合适的含量测定方法。方法：采用HPLC同时测定抱茎獐牙菜中獐牙菜苦苷、龙胆苦苷、芒果苷、獐牙菜苷、齐墩果酸的含量。以甲醇-0．02％磷酸为流动相，梯度洗脱，资生堂UGl20 C_18色谱柱（4．6mm×250mm，5μm），柱温25℃，流速1．0mL·min-1，检测波长243nm。结果：獐牙菜苦苷、龙胆苦苷、芒果苷分别在1．50～18．04，0．37～4．49，0．06～0．70μg线性关系良好，r均为1．0000。平均加样回收率分别为96．3％，99．0％，101．0％。结论：獐牙菜苦苷、龙胆苦苷、芒果苷3种成分在抱茎獐牙菜中的分离度良好，检出量合适。选定的方法重复好，结果准确，可作为抱茎獐牙菜质量标准中含量测定的方法，并为区别其他獐牙菜，合理利用开发抱茎獐牙菜资源提供重要参考。

Objective： To establish an appropriate method in standard control for determination the content of Swertiafranchetiana H. Smith. Method： Simultaneous determination of swertiamarin, gentiopicroside, mangiferin, sweroside and oleanolic acid in S. franchetiana intends to HPLC method. Eluted with methanol-0.02 % phosphoric acid at a flow rate of 1.0 mL · min-1, Shiseido UG120 Cls （4.6 mm ×250 mm, 5 μm） column, the detection wavelength was 243 nm, and the column temperature was 25 ℃. Result： The linear range of swertiamarin, gentiopicrin and mangiferin was with in the range of 1.50-18.04, 0. 37-4.49, 0.06-0.70 μg, r = 1.000 0. The average recoveries were 96.3%, 99.0%, 101.0%. Conclusion： Swertiamarin, gentiopicroside and mangiferin have good degrees of separation and proper detection of amount. The selected method is reproducible, accurate, and can be used for determination of S. franchetiana in quality standard, and the difference between other Swertia, provide important reference for the reasonable utilization of resources and development.