豆丹对D-半乳糖致衰老小鼠的抗衰老作用

Anti-aging Effects of Clanis bilineata tsingtauica in Aging Mice Induced by D-galactose

目的:D-半乳糖sc建立亚急性衰老小鼠模型,研究豆丹的抗衰老作用。方法:将实验动物随机分为7组,分别为正常对照组、衰老模型组、西洋参组(0.5 g·kg-1)、豆丹水提液高(2.8 g·kg-1)、低剂量组(1.4 g·kg-1)、豆丹醇提液高(12.5 g·kg-1)、低剂量组(6.25 g·kg-1)。造模14 d后开始给药,给药14 d后测定负重游泳时间、脏器系数、肝脏超氧化物歧化酶(SOD)活性、肝脏及脑组织谷胱甘肽过氧化物酶(GSH-Px)活性、肝及脑组织丙二醛(MDA)含量。结果:与正常组比较,衰老模型组负重游泳时间、脏器指数、SOD及GSH-Px均降低,MDA含量增加,且均有显著性差异(P<0.05或P<0.01);与衰老模型组比较,豆丹水提液、醇提液均能显著延长负重游泳时间(P<0.05或P<0.01),提高脏器指数(P<0.05),提高SOD,GSHPx活性(P<0.05或P<0.01),降低MDA含量(P<0.05),且对雌雄小鼠的作用大体相当。结论:豆丹可能通过提高机体免疫、抗脂质过氧化和清除氧自由基的能力来发挥抗衰老的作用。

Objective： Anti-aging effect of Clanis bilineata tsingtauica was investigated through the model of subacute aging mice induced by subcutaneous injection of D-galactose in the study. Method： The Kunming mice were divided into seven groups： normal control group, aging model group, Panax quinquefolius positive control group, low and high-dose C. bilineata tsingtauica＇s water extraction group （1.4, 2.8 g ·kg-1） , low and high-dose Clanis bilineata tsingtauica＇s 75% ethanol extraction （6.25, 12.5 g ·kg-1 ）. 14 days after the mice were sc given D-galactose, the corresponding drugs were ig given to the mice, respectively. After 14 days, the loaded swimming time, the viscera index, superoxide dismutase （SOD） activities in liver homogenate, glutathione peroxidase （GSH-Px） activities in liver and brain homogenate and malondialdehyde （MDA） content in liver and brain homogenate were measured. Result： Compared with the normal control group, the loaded swimming time, the viscera index, the T-SOl） activities and the GSH-Px activities in liver and brain were markedly reduced in aging model group, while the content of MDA was markedly increased （P 〈 0.05 or P 〈 0.01 ）. Both water extraction and 75 % ethanl extraction of C. bilineata tsingtauica could significantly extend the loaded swimming time （P 〈 0. 05. or P 〈 O. 01 ）, increase the viscera index （P 〈 0. 05）, elevat the activities of T-SOD and GSH-Px and decrease the contents of MDA （ P 〈 0.05 or P 〈 0.01 ） , and the effects on male and female are generally the same. Conclusion： C. bilineata tsingtauica has an anti-aging effect by improving immune and antioxidant capacity.