葶苈草颗粒对恶唑酮诱导的小鼠实验性肠炎的治疗作用

Therapeutic Effect of Pepperweed Seed for Oxazolone-induced Colitis in Mice

目的:观察葶苈草颗粒对炎症性肠病(IBD)的治疗作用并探讨其作用机制。方法:选昆明种小鼠,用3%恶唑酮(OXZ)复制小鼠OXZ结肠炎模型。实验分为正常对照、模型对照组(等容蒸馏水)、葶苈草颗粒(1.95,3.9,7.8 g·kg-1)治疗组、柳氮磺胺吡啶(0.39 g·kg-1)阳性药对照组,ig每天1次,连续治疗4 d。用酶联免疫吸附(ELISA)法测定血清和结肠组织中白细胞介素-10(IL-10)、结肠组织中髓过氧化物酶(MPO)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的含量,进行疾病活动指数(DAI)评分和病理组织学观察。结果:治疗4 d后,与模型组比较,葶苈草颗粒3.9,7.8 g·kg-1治疗组,血清和结肠组织IL-10含量明显升高(P<0.05或P<0.01),结肠组织MPO,IL-1β,TNF-α和IL-6含量明显降低(P<0.05或P<0.01);葶苈草颗粒治疗组总DAI值明显减少(P<0.05或P<0.01)。结论:葶苈草颗粒对OXZ诱导的小鼠实验性肠炎具有较好的治疗作用,其可能通过调节促炎细胞因子IL-1β,TNF-α,IL-6和抑炎细胞因子IL-10之间的平衡,有效减轻实验性结肠炎炎症,从而起到治疗IBD的作用。

Objective： To explore the therapeutic effect of pepperweed seed on inflammatory bowel disease （IBD） and the underlying mechanism. Method： The IBD mice model was induced by oxazolone （OXZ）. Fifty modeling mice were randomly divided into 5 groups ： model group, sulfasalazine （ SASP, 0.39 g · kg- 1 ） group and pepperweed seed （1.95, 3.9, 7.8 g·kg-1） groups, 10 mice each group. Another 10 mice were selected as normal group. The modeling mice were orally treated daily for 4 days. The normal group and the model group received distilled water for 4 days. The contents of interleukin （IL） -10 in serum and colon were detected by enzyme-linked immunosorbent assay （ELISA）. Similarly, the contents of myeloperoxidase （MPO） , interleukin IL-1β, IL-6 and tumor necrosis factor-α （TNF-a） in colon tissues were detected. The disease activity index （DAI） and histological changes of colon were evaluated. Result： After 4 days＇ treatment, compared with the model group, the contents of interleukin 10 （IL-10） in serum and colon tissues in pepperweed seed （3.9, 7.8 g · kg- 1 ） groups were significantly increased （ P 〈 O. 05, P 〈 O. O1 ）, the contents of MPO, 1β（ IL-1β）, IL-6 andTNF-α in colon tissues in pepperweed seed （3.9, 7.8 g ·kg-1） groups were significantly reduced. The total DAI level in pepperweed seed groups were significantly decreased （P 〈 0.05, P 〈 0.01 ）. Conclusion： The pepperweed seed exerted therapeutic effect on oxazolone-induced colitis in mice, which is related to the regulation of IL-1β, TNF-α, IL-6 and IL-10. It can alleviate oxazolone-induced colitis and be valuable in the IBD treatment.