基于石墨烯/金纳米复合材料的无酶葡萄糖传感器

Nonenzymatic Glucose Sensor Based on Graphene/Gold Nanohybrids

以抗坏血酸（AA）为还原剂，通过一步还原法将氧化石墨烯和氯金酸同时还原，合成石墨烯／金纳米复合材料，并直接滴涂于玻碳电极表面，构建基于石墨烯／金纳米复合材料的无酶葡萄糖传感器。采用循环伏安法和线性扫描伏安法对传感器的性质进行了研究。结果表明，该传感器能催化葡萄糖的氧化，且其氧化峰电流随葡萄糖浓度的增大而增大。测定葡萄糖的线性范围为0．01～2．5mmol／L（R=0．9964），检出限（s／N=3）为3μmol／L。对同一浓度的葡萄糖溶液平行测定8次，其电流强度的相对标准偏差（RSD）为2．6％。该传感器制作简单、稳定性好，将其用于葡萄糖注射液的检测，方法灵敏，其加标回收率为92．9％。

Graphene/gold nanohybrids （Gra/Au NHs） were synthesized by a facile chemical reduction method using L-ascorbic acid（L-AA） as reducing agent, and novel nonenzymatic glucose sensor was prepared by modifying this nanohybrids on glassy carbon electrode. The presented sensor showed prominent electrocatalytic activity towards the oxidation of glucose, which could be attributed to the remarkable synergistic effect of the Gra/Au NHs. The linear relationship between concentration oxidation current and the glucose concentration was observed to be from 0. 01-2. 5 mmol/L with a detection limit of 3 μmol/L（S/N=3）. Futhermore the sensor showed good reproducibility for glucose detection and the relative standard deviation was 2.6% for 1 mmol/L glucose（n=8）. The major merit of the proposed sensor lies on its simple fabrication, high sensitivity and good reproducibility. The presented sensor was successfully applied for the determination of glucose in glucose injection with the recovery of 92.9%.