荧光免疫夹心法分离及检测EV71病毒

Fluoroimmunoassay with Double-antibody Sandwich for the Separation and Detection of EV71 Virus

基于免疫磁球分离技术，利用荧光免疫夹心法捕获，建立了一种新的EV71病毒的快速检测方法。用免疫磁球特异性捕获EV71病毒，然后用生物素化的抗体结合EV71病毒，再结合标有量子点的链霉亲和素（SA—QDs）形成复合物，采用荧光光谱法进行定量检测，C4亚型EV71病毒在2．6～7．51g（TCID50／mL）的范围内线性关系良好（R2=0．9953），检测限为1．71g（TCID50／mL）。该方法简便快速，灵敏度高。

In this experiment,a new method for rapid detection of EV71 virus was established based on immunomagnetic beads（IMB） separation and fluoroimmunoassay with double-antibody sandwich. EV71 virus was captured by IMB, and then connected with biotinylated antibodies and combined with streptavidin-quantum dots （SA-QDs）. The complexes were quantitatively detected by fluorescence spectrometer. It has a good linear relationship within 2. 6-7. 5 lg（TCID50/mL）（R2 =0. 9953） and a detection limit of 1.7 lg（TCID50/mL）. The method is simple and rapid.