摘要
以C18固相萃取(SPE)小柱净化样品,富集苏丹红,并基于苏丹红Ⅰ~Ⅳ对鲁米诺一KIO。体系发光强度的增敏作用,建立了同时测定苏丹红染料的新方法。实验采用PursuitC18色谱柱(250×4.6mm,i.d.,5gm),以乙腈~水体系为流动相进行梯度洗脱,在0.001~0.50gg/mL范围内,苏丹红工~Ⅳ的浓度与发光强度呈良好的线性关系,相关系数在0.9981以上,苏丹红工~Ⅳ的检出限分别为0.3、0.2、0.3、0.2ng/mL,加标回收率在90.0%~96.7%之间。该方法能有效地去除基质干扰,具有简单、快速、灵敏的特点,适用于苏丹红染料的分析检测。
In this paper,a new method for the determination of sudan dyes was estalished based on the sensitizing effect of Sudan dyes on luminal-KIO4 chemilminescenee system. The results showed that there was a good linear relationship between the CL intensities and the concentrations of Sudan dyes in the range of 1.0 ng/mL-0. 50 /,g/mL with the correlation coefficients more than 0. 9981. The detection limits of Sudan I -1g were 0.3,0.2,0, 3 and 0.2 ng/mL respectively. The recoveries were in the range of 90.0%- 96. 7%. The method has been proved to he excellent for sample purification, and simple, sensitive and accurate in the analysis of Sudan dyes.
出处
《分析科学学报》
CAS
CSCD
北大核心
2014年第3期401-404,共4页
Journal of Analytical Science
关键词
固相萃取
高效液相色谱
化学发光
鲁米诺
苏丹红染料
Solid-phase extraction(SPE)High performance liquid chromatography(HPLC)
Chemiluminescence(CL)
Luminol
Sudan dyes