固相萃取-高效液相色谱化学发光法测定食品中微量苏丹红染料

Determination of Sudan Dyes in Food by Solid-phase Extraction and High Performance Liquid Chromatography-Chemiluminescence Method

以C18固相萃取（SPE）小柱净化样品，富集苏丹红，并基于苏丹红Ⅰ～Ⅳ对鲁米诺一KIO。体系发光强度的增敏作用，建立了同时测定苏丹红染料的新方法。实验采用PursuitC18色谱柱（250×4．6mm，i．d．，5gm），以乙腈～水体系为流动相进行梯度洗脱，在0．001～0．50gg／mL范围内，苏丹红工～Ⅳ的浓度与发光强度呈良好的线性关系，相关系数在0．9981以上，苏丹红工～Ⅳ的检出限分别为0．3、0．2、0．3、0．2ng／mL，加标回收率在90．0％～96．7％之间。该方法能有效地去除基质干扰，具有简单、快速、灵敏的特点，适用于苏丹红染料的分析检测。

In this paper,a new method for the determination of sudan dyes was estalished based on the sensitizing effect of Sudan dyes on luminal-KIO4 chemilminescenee system. The results showed that there was a good linear relationship between the CL intensities and the concentrations of Sudan dyes in the range of 1.0 ng/mL-0. 50 /,g/mL with the correlation coefficients more than 0. 9981. The detection limits of Sudan I -1g were 0.3,0.2,0, 3 and 0.2 ng/mL respectively. The recoveries were in the range of 90.0%- 96. 7%. The method has been proved to he excellent for sample purification, and simple, sensitive and accurate in the analysis of Sudan dyes.