共振散射法测定安乃近含量

Determination of Analginum by Resonance Scattering Method

在pH=5．0的B—R缓冲溶液中，Fe（Ⅲ）与邻菲哆啉反应生成无色配合物，从而使得体系在374nm波长处产生1个较强的共振散射峰。加入安乃近（或4-甲氨基安替比林）后，其将Fe（Ⅲ）还原成Fe（Ⅱ），而生成一种橙红色配合物，使得体系在374nm（或373nm）波长处的共振散射信号减弱。在最佳实验条件下，当安乃近浓度在0．634～15．2μg／ml范围内，体系的共振散射信号△I与安乃近的浓度之间有较好的线性关系，检出限为5．75ng／mL。该方法可直接用于测定安乃近含量，回收率为100．4％～103．4％。

In pH = 5. 0 B-R buffer solution, Fe （Ⅲ） reacted with phenanthroline to yield colorless complexes, which produced a strong resonance scattering peak at 374 nm. With the hddition of analginum （or 4-Novalgin）, Fe（Ⅲ ） was reduced to Fe（ Ⅱ ） to form orange-red complexes, and the resonance scattering signal of the systems was weakened at 374 nm （or 373 nm）. Under the optimum experimental conditions, there was a good linear relationship between the resonance scattering signal and the concentration of analginum in the range of 0. 634-15.2 μg/mL with a detection limit of 5.75 ng/mL. This method could be directly used for the determination of analginum with recoveries of 100.4 %-103.4%.