硝普钠分光光度法测定头孢克肟

Spectrophotometric Determination of Cefixime Using Sodium Nitroprusside

研究了硝普钠（SNP）与头孢克肟（CEF）之间的显色反应机理，确定了最佳反应条件，建立了一种测定CEF的新方法。结果表明：在NaOH溶液浓度为0．17mol／L的碱性介质中，控制温度40℃，反应30rain，CEF与SNP以1：2化学计量比生成一种含亚硝基的红褐色化合物，最大吸收波长为530nm，表观摩尔吸光系数ε=1．96×103^L／（mol·cm）。CEF的浓度在0．8～400μg／mL范围内服从比耳定律，线性相关系数为0．9991，检测限（3σ／κ）为0．48μg／mL。

The chromogenic reaction of cefixime（CEF） and sodium nitroprusside（SNP） was investigated by spectrophotometry. A method for the determination of CEF has been developed. A colored product was formed by the reaction of CEF and SNP with the reaction stoichiometric ratio of 1：2 in an alkaline solution. The reaction time was 30min at 40 ℃. The apparent molar absorptivity was 1.96 × 10s L/mol. cm at the maximum absorption wavelength of 530 nm. Beer＇s law was well obeyed when CEF concentration was in a range of 0.8-400 μg/mL with a correlation coefficient of 0. 9991 and the detection limit （3σ/k） was 0.48 μg/mL.