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转化生长因子β_1对兔角膜基质细胞胶原降解代谢的影响 被引量：1

Effect of TGF-β on collagen degradation of rabbit keratocytes
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摘要 目的 :观察转化生长因子β1对角膜基质细胞胶原降解作用的影响。方法 :角膜基质细胞三维胶原凝胶表面分别添加含纤溶酶原 ( 10 0 mg/ L)及不同浓度 ( 0、0 .1、1.0和 10 .0μg/ L )转化生长因子β1的MEM液 ,培养 2 4 h,测定培养液中羟脯氨酸含量做为胶原降解活性。结果 :转化生长因子β1以剂量依赖关系减少角膜基质细胞胶原降解。结论 :转化生长因子β1抑制角膜基质细胞胶原降解 ,可能有助于角膜溃疡的治疗。 Objective:To observe the effect of TGF-β 1 on collagen degradation of rabbit keratocytes. Methods:The activity of collagen degradation of keratocytes with three-dimensional culture in collagen gel was determined with spectrophotometer.Results:TGF-β inhibited collagen degradation of keratocytes in dose-dependent manner.Conclusion:The present result suggests that TGF-β 1 has the inhibitory effect on collagen degradation of keratocytes and may help in the treatment of corneal ulceration. [
作者 李云 张冰洁 尹正玉 刘伯明 郝继龙 贾卉 张吉豫
机构地区 吉林大学中日联谊医院眼科 延吉市医院眼科 吉林省南湖医院
出处 《白求恩医科大学学报》 CAS CSCD 北大核心 2001年第2期153-155,共3页 Journal of Norman Bethune University of Medical Science
关键词 转化生长因子Β1 角膜基质细胞 胶原降解 角膜溃疡 transforming growth factor-β 1 keratocyte collagen degradation
分类号 R772.210.2 [医药卫生—眼科]
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白求恩医科大学学报
2001年 第2期

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