脑络通颗粒对动脉粥样硬化小鼠血管组织NF-κB、PPAR-γ的影响

Effects of Naoluotong Granules on NF-κB and PPAR-γ in Vascular Tissue of Atherosclerotic Mice

目的探讨脑络通颗粒抗动脉粥样硬化(AS)的可能作用机制。方法 30只8周龄ApoE-/-小鼠高脂饲料饲养10个月建立AS模型,随机分为模型组、脑络通组、辛伐他汀组,每组10只;同时以同周龄C57BL/6J小鼠10只作为空白组。脑络通组小鼠给予脑络通颗粒溶液6.67 g/kg灌胃,辛伐他汀组给予辛伐他汀片溶液6.67mg/kg灌胃,空白组和模型组给予等量生理盐水,各组均每天灌胃1次,共2个月。比较各组小鼠血脂水平及血管组织斑块面积指数、肿瘤坏死因子α(TNF-α)、核因子κB(NF-κB)、过氧化体增殖物激活型受体γ(PPAR-γ)蛋白及mRNA水平。结果与空白组比较,模型组小鼠血脂水平及血管组织TNF-α蛋白、NF-κB蛋白及mRNA升高,PPAR-γ蛋白及mRNA降低(P<0.01);与模型组比较,脑络通组和辛伐他汀组小鼠血脂水平、血管组织斑块面积指数及血管组织TNF-α蛋白、NF-κB蛋白及mRNA降低,PPAR-γ蛋白及mRNA升高(P<0.01)。结论脑络通颗粒能调控ApoE-/-小鼠AS模型NF-κB与PPAR-γ的平衡,可能是其抗AS的机制之一。

Objective To study the possible mechanism of Naoluotong Granules for atherosclerosis （AS）. Methods The AS models were established by high-fat diet feeding 30 8-week-old ApoE-/- mice for 10 months. The model mice were randomized into the model group, Naoluotong group and Simvastatin group, with 10 in each. Ten 8-week-old C57BL/6J mice were selected as the blank group. The mice in the Naoluotong group were gavaged with 6. 67g/kg Naoluotong Granules solution. The mice in the Simvastatin group were gavaged with 6. 67mg/kg Simvastatin Tablets solution. The mice in the blank group and model group were gavaged with the same amount of normal saline.The mice in each group were given intragastric administration once daily for 2 months. The levels of blood lipids, vascular tissue plaque area index, tumor necrosis factor-α （TNF-α） , nuclear factor-κB （NF-κB） and peroxisome proliferator-activated receptor-γ （PPAR-γ） were compared. Results Comparing with the blank group, the levels ofblood lipids, TNF-ot and NF-κB were significantly increased but the PPAR-γ level was significantly decreased in the model group （P 〈 0. 01 ）. Comparing with the model group, the levels of blood lipids, vascular tissue plaque area index, TNF-α and NF-κB were significantly decreased but the PPAR-γ level was significantly increased in the Naoluo-tong group and Simvastatin group （P 〈 0. 01 ）. Conclusion Naoluotong Granules is effective for AS, which may be relevant to regulating the balance of NF-κB and PPAR-γ in ApoE-/- mouse models.