促红细胞生成素对心肺复苏后大鼠神经功能及脑细胞凋亡的影响

Effects of erythropoietin on neural function and brain cell apoptosis in rats after cardiopulmonary resuscitation

目的 探讨促红细胞生成素（EPO）对心肺复苏后大鼠神经功能及脑细胞凋亡的影响.方法 将成年雄性SD大鼠随机分为对照组和EPO组,每组24只,采用窒息法制备心肺复苏模型,神经功能缺损评分（NDS）法评价复苏后12 h、24 h大鼠的神经功能,于复苏后0h、12 h、24 h用RT-PCR法检测大脑皮层组织凋亡诱导因子（AIF）、caspas-3 mRNA的水平.结果 EPO组心肺复苏后12 h、24h NDS评分分别为（70.50±4.04）分和（65.88±2.64）分,与对照组[12 h：（60.00±3.38）分;24h：（54.50±2.56）分]比较,差异有统计学意义（P＜0.01）;EPO组AIF基因mRNA表达水平在复苏后12h和24 h分别为（1.31±0.26）、（1.87±0.17）,与对照组[12h：（1.88±0.18）;24h：（2.71±0.24）]比较显著降低（均P＜0.01）;EPO组caspase-3基因的mRNA表达水平在复苏后12h和24 h分别为（1.49±0.15）、（1.56±0.10）,与对照组[12 h：（1.68±0.10）;24h：（1.84±0.16）]比较显著降低（均P＜0.01）;结论 EPO可降低AIF、caspase-3 mRNA的转录,减少脑缺血和再灌注损伤导致的皮层神经细胞凋亡,改善心肺复苏后大鼠的脑功能.

Objective To study the effect of erythropoietin （EPO） on neural function and brain cell apoptosis in rats after cardiopulmonary resuscitation.Methods Adult male SD rats were randomly divided into control group and EPO group with 24 in each group.A rat model of asphyxial cardiac arrest and cardiopulmonary resuscitation was established.The neurological functions were assessed using neurological deficit score （NDS） 12 h and 24 h after cardiopulmonary resuscitation.The expressions of the apoptosis-inducing factor （AIF） and caspase-3 mRNA in cerebral cortex tissue were detected using reverse transcription-polymerase chain reaction （RT-PCR） at 0 h,12 h,and 24 h,respectively.Results Compared with the DNS scores in the control group （12 h：（60.00± 3.38） ;24 h：（54.50±2.56）,respectively）,12 h and 24 h NDS scores were （70.50±4.04） and （65.88±2.64） in EPO group after cardiopulmonary resuscitation,and the difference was statistically different （P＜0.01）.The AIF mRNA expression levels of 12 h （1.31±0.26） and 24 h （1.87±0.17） after cardiopulmonary resuscitation in EPO group were obviously lower than those in the control group （12 h：（1.88 ± 0.18）,24h：（2.71 ± 0.24）,respectively）,and the differences were statistically different （P＜0.01）.The Caspas-3 mRNA expression levels of 12 h （1.49± 0.15） and 24 h （1.56±0.10） after cardiopuhmonary resuscitation in EPO group were obviously lower than those in the control group （12 h：（1.68± 0.10）,24h：（1.84 ± 0.16）,respectively）,and the differences were statistically significant （P＜0.01）.Conclusion EPO can reduce AIF and caspase-3 mRNA transcription,reduce apoptosis in cortical neurons caused by the cerebral ischemia and reperfusion injury after cardiopulmonary resuscitation,and therefore improve brain function.