SD大鼠转化生长因子α基因cDNA序列的分子克隆

Molecular Cloning of cDNA Sequences Encoding TGFα Gene in the SD Rats

试验参考了SD大鼠转化生长因子α(transforming growth factor alpha,TGFα)基因序列,用PCR方法对SD大鼠TGFα基因cDNA序列进行了克隆,获得了SD大鼠TGFα基因的970bp的cDNA序列,提交GenBank,登录号:KF366251,其中CDS长度为483bp,共编码了160个氨基酸。SD大鼠与BN大鼠、小家鼠、黄牛、野猪、猕猴、黑猩猩、人、原鸡、鹌鹑和斑马鱼的TGFα基因编码序列的同源性分别为98.6%、95.0%、85.5%、85.3%、85.5%、86.3%、86.3%、73.2%、73.2%和56.8%,其编码蛋白的氨基酸序列同源性分别为98.8%、96.9%、90.6%、90.6%、91.2%、91.9%、91.9%、72.6%、72.6%和38.8%。这一结果表明了TGFα基因在进化过程中具有一定的保守性,但不同物种之间也具有功能上的特异性。通过比较SD大鼠与BN大鼠的TGFα基因编码序列,发现了7个SNPs位点,这一结果为TGFα基因的SNPs数据库提供了新的信息。

This study was designed to clone and analyze the cDNA encoding TGFa from SD rats. The PCR method was developed to clone the TGFα cDNA. A full-length cDNA and CDS sequences of SD rat were 970 and 483 bp, which had been accepted by GenBank （accession number.. KF366251）. TGFα protein encoded by this gene was composed of 160 amino acid residues. The identities of coding sequences of TGFα gene were 98.6%, 95.0%, 85.5%, 85.3%, 85.5%, 86.3%, 86.3%, 73.2%, 73.2% and 56.8% by homologous comparison among SD rat and other species, and in amino acid sequences were 98.8%, 96.9%, 90.6%, 90.6%, 91.2%, 91.9%, 91.9%, 72.6%, 72.6% and 38.8%, respectively. The results suggested a certain degree of conservation of the TGFα gene among different species, but there were some specificity in function among different species. The results derived from information searching by BLAST program revealed there were 7 SNPs sites in the sequences of TGFa gene coding sequences between SD rats and BN rats in GenBank. The results provided new data for SNPs in TGFα gene.