转Bt基因糙米作为日粮原料对生长猪免疫功能的影响

Effect of Transgenic Bt Brown Rice as the Dietary Ingredients on Immunity in Growing Pigs

本试验旨在研究转苏云金芽孢杆菌(Bacillus thuringiensis,Bt)基因糙米作为日粮原料对生长猪免疫功能的影响。选用80日龄大白仔猪30头,随机分为2组,每组15个重复,每个重复1头猪。对照组饲喂含70%亲本非转基因糙米的日粮,试验组饲喂含70%转Bt基因糙米的日粮,试验期90d。结果显示,与对照组相比,饲喂转Bt基因糙米的生长猪血清中IgA、IgG、IgM含量及补体C3和C4水平差异均不显著(P>0.05);试验组生长猪的肝脏、脾脏组织切片观察发现,其亚显微结构均无病理变化,与对照组相比无明显差异(P>0.05),且两组间的免疫器官指数也均无显著差异(P>0.05)。综上所述,在本试验条件下,转Bt基因糙米作为生长猪的日粮,未见对生长猪的免疫器官及其功能造成影响。

The experiment aimed to study the effect of transgenic Bt brown rice as growing pig＇s diet on immunity in grow ing pigs. 30 Large White piglets were randomly allocated into 2 dietary treatments with 15 replicates per treatment. The control group diet consisted of basic diet and 70% non-transgenic Bt brown rice, and the test group consisted of basic diet and 70% transgenic Bt brown rice. This experiment lasted for 90 days. The results showed that effect of transgenic Bt brown rice on IgA,IgG,IgM contents and complements C3 and C4 levels in serum were no significant differences between the two groups （P〉0. 05）. Submicrostructure of liver and spleen of growing pigs in treatment group had no pathological changes according to tissue slice observation of two organs and also had no significant difference compared with the control group （P〉0.05）. And there were no significant differences in immune organ index between the two groups （P〉0.05）. In conclusion, under this experimental condition,Bt brown rice as growing pig＇s diet fed to growing pigs will not cause damage to immune organs and will not influence immune function.