3种猪繁殖与呼吸综合征病毒抗体检测试剂盒的比较与应用

Comparison of Three ELISA Kits for the Detection of Porcine Reproductive and Respiratory Syndrome Virus Antibody and its Application

本研究采用3种不同的猪繁殖与呼吸综合征病毒(procine reproductive and respiratory syndrome virus,PRRSV)抗体检测试剂盒对仔猪母源抗体和免疫猪抗体消长规律进行了比较分析,以期为猪群PRRSV抗体监测提供试剂盒选择依据。将10头PRRSV、PCV2病原双阴性仔猪随机分为2组,每组5头,2组仔猪分别于14日龄及PRRSV母源抗体转阴后免疫高致病性猪繁殖与呼吸综合征活疫苗,于14、21、28、35、42、49、56、70、84、98日龄采血并分离血清,分别用3种商品化PRRSV抗体检测试剂盒检测抗体水平,同时检测母猪分娩后14d抗体水平;结果显示,试剂盒A与试剂盒C的Kappa值=0.784>0.6,试剂盒A与试剂盒C高度一致;试剂盒B与试剂盒A、试剂盒B与试剂盒C的Kappa值分别为0.422、0.374,试剂盒B与试剂盒A一致性中度,试剂盒B与试剂盒C一致性较弱。母源抗体监测表明,3种试剂盒检测的PRRSV母源抗体均在42日龄左右转阴。试剂盒A与试剂盒C所检测的PRRSV相关抗体在免疫后1周即产生,3周即可达到峰值,而试剂盒B所检测的PRRSV相关抗体在免疫后3周产生,5周达到峰值。本研究为3种试剂盒对临床PRRSV的抗体监测和免疫评估提供了试验依据。

To comparatively analyzed piglets maternal antibodies and the antibody dynamics in immunized pigs using three different of antibody detection Kits of porcine reproductive and respiratory syndrome virus （PRRSV） ,in order to provide selection refereace for Kits of monitoring PRRSV antibodies of pigs. The results showed that the Kappa value = 0. 784〉0. 6 between Kit A and Kit C,the compared results were highly-consistent^the Kappa value was 0. 422 between Kit B and Kit A,the Kappa value was 0. 374 between Kit B and Kit C. Maternal antibody monitoring showed that PRRSV maternal antibodies of three Kits had become negative at 42 days old. PRRSV associated antibodies of using Kit A and Kit C were detected after immuned one week, three weeks to reach the peak, while the PRRSV antibodies of using Kit B were detected three weeks after immunization, and reached the peak in five weeks. This study provided the experimental basis for the three Kits were used to antibody surveillance and immune assessment in the clinical PRRSV.