c-myc反义寡核苷酸对白血病细胞凋亡的影响

Study on the Apoptosis of Leukemic Cell Induced by Antisense Oligodeoxynucleotides Complementary to c myc

目的 研究c myc反义寡核苷酸对白血病细胞凋亡的影响。方法 用针对c mycmRNA 5′端非翻译区的一个 18bp的反义寡核苷酸与HL6 0细胞、分离的急性粒细胞白血病病人白细胞共培养 10h ,用凋亡细胞计数、DNA凝胶电泳、ELISA、流式细胞仪等方法检测白血病细胞的凋亡。结果 c myc反义寡核苷酸能够诱导HL6 0细胞凋亡 ,且此作用随着作用浓度的提高而增加 ;此反义核酸只诱导白血病患者的白血病细胞凋亡 ,对正常白细胞的凋亡无影响。结论 c myc反义核酸可以通过诱导白血病细胞凋亡来治疗急性粒细胞白血病 。

Purpose: To observe the effect of Anti-c-myc on the apoptosis of HL60 cells and leukemic patients'leukocytes. Methods: HL60 cells and leukemic patients, leukocytes were treated with anti-c-myc oligomer-5′d(CTT CTC GAG GCA GGA GGG)3′-complementary to the transcriptional initiation of c-myc mRNA. After 10 hours, we detected the apoptosis by several methods: DNA agarose gel electrophoresis, ELISA(enzyme - linked immunosorbent assay), Flow Cytometric Analysis, and Accounting the number of apoptotic cells after stained with Wright's. Results: The apoptosis of HL60 cells were induced by the anti-c-myc oligomer, and the induction of apoptosis was dose-dependent, i. e. more HL60 cells were apoptotic as we treated them with higher dose of the antisense oligodeoxynucleotides. Furthermore, the anti-c-myc oligomer also induced the apoptosis of leukemic patients, leukocytes, but had no effect on the apoptosis of normal leukocytes. Conclusions: These results imply that we can treat acute myeloid leukemia with the anti-c-myc oligomer through apoptosis-inducing, and it has good specificity. This study provides basis for leukemia treatment.